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GUCA2A recombinant protein :: Proguanylin Recombinant Protein

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Catalog # MBS142674
Unit / Price
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  0.002 mg  /  $140 +1 FREE 8GB USB
  0.01 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $3,900 +4 FREE 8GB USB
GUCA2A recombinant protein
Product Name

Proguanylin (GUCA2A), Recombinant Protein

Full Product Name

Recombinant Human Proguanylin

Product Synonym Names
Proguanylin Human; Proguanylin Human Recombinant; Guanylin Precursor; Guanylate cyclase activator 2A; Guanylate cyclase-activating protein 1; Gap-IGUCA2; STARA; GUANYLIN; Proguanylin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MKHHHHH HAS VTVQDGNFSF SLESVKKLKD LQEPQEPRVG KLRNFAPIPG EPVVPILCSN PNFPEELKPL CKEPNAQEIL QRLEEIAEDP GTCEICAYAA CTGC
OMIM
139392
3D Structure
ModBase 3D Structure for Q02747
Host
E Coli
Specificity
The proguanylin human recombinant is 100% homologous with the human proguanylin.
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Three-step procedure using IMAC and size exclusion chromatography before and after refolding.
Form/Format
Sterile filtered and lyophilized from 0.5 mg/ml in deionized H2O.
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
Preparation and Storage
Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.
Other Notes
Small volumes of GUCA2A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GUCA2A recombinant protein
Description: Proguanylin Human Recombinant produced in E Coli, is an 11 kDa protein containing 96 amino acid residues of the human proguanylin and 2 additional amino acid residues (underlined).The Proguanylin is purified by proprietary chromatographic techniques.GPVTVQDGNF SFSLESVKKL KDLQEPQEPR VGKLRNFAPI PGEPVVPILC SNPNFPEELK PLCKEPNAQE ILQRLEEIAE DPGTCEICAY AACTGC.

Introduction: Heat-stable enterotoxins (STa) are small, cysteine-rich peptides secreted by Escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as STaR. Binding of STa to STaR induces a dramatic increase in the cGMP content of the cell; the increase, in turn, inhibits salt absorption and stimulates chloride secretion. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to the diarrhea and dehydration characteristic of enterotoxin activity. The identification of a receptor for STa on intestinal brush border membranes suggested the existence of an endogenous activator, described guanylin, a 15-amino acid peptide purified from rat small intestine, as a potential ligand for the STaR. This peptide shares sequence similarity with STa; see also uroguanylin. The molecular cloning of the human and mouse cDNAs encoding guanylin was reported. The sequences demonstrated that guanylin is present at the C-terminal end of a larger precursor protein. Expression in mammalian cells indicated that the 94- amino acid proguanylin is inactive. The biologically active guanylin can be released by either chemical or enzymatic treatment of proguanylin. By Northern blot analysis and in situ hybridization, showed that expression of guanylin mRNA is restricted to cells of the intestinal epithelium, specifically the Paneth cells at the base of the small intestinal crypts. These results demonstrate that guanylin is an endogenous activator of STaR isolated a cDNA encoding an apparent precursor of guanylin from a human intestinal cDNA library. The mRNA was expressed at high levels in human ileum and colon. In the mouse, interspecific backcross analysis used to map the Guca2 gene to the distal half of mouse chromosome 4 in a region of homology with human chromosome 1p. By fluorescence in situ hybridization mapped the GUCA2 gene to human 1p35-p34 Guanylin is thought to modulate intestinal water/electrolyte transport in a paracrine mode reported the nucleotide sequence of the gene, the characteristics of its circulating molecular form, and its localization in enterochromaffin cells of the gut. The gene, approximately 2.6 kb in size, consists of 3 exons interrupted by 2 introns. The hormonal form of guanylin is a 94-amino acid peptide with a molecular mass of 10.3 kDa. Guanylin is synthesized by gut enterochromaffin cells as a prohormone of 115 amino acids and is processed to the molecular form of 94 amino acids circulating in the blood.
Product Categories/Family for GUCA2A recombinant protein
NCBI/Uniprot data below describe general gene information for GUCA2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
12,388 Da[Similar Products]
NCBI Official Full Name
guanylin
NCBI Official Synonym Full Names
guanylate cyclase activator 2A (guanylin)
NCBI Official Symbol
GUCA2A  [Similar Products]
NCBI Official Synonym Symbols
GUCA2; STARA; GCAP-I
  [Similar Products]
NCBI Protein Information
guanylin; guanylate cyclase activator 2A (guanylin 2, intestinal, heat-stable); guanylate cyclase-activating protein 1; guanylate cyclase-activating protein I; prepro-guanylin
UniProt Protein Name
Guanylin
UniProt Synonym Protein Names
Guanylate cyclase activator 2A; Guanylate cyclase-activating protein 1; Guanylate cyclase-activating protein I; GCAP-I
Protein Family
UniProt Gene Name
GUCA2A  [Similar Products]
UniProt Synonym Gene Names
GUCA2; GCAP-I  [Similar Products]
UniProt Entry Name
GUC2A_HUMAN
UniProt Comments for GUCA2A
GUCA2A: Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. Belongs to the guanylin family.

Protein type: Activator; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p35-p34

Cellular Component: extracellular region

Molecular Function: hormone activity; guanylate cyclase activator activity

Biological Process: positive regulation of guanylate cyclase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with GUCA2A recombinant protein
 Reference Product  PubMed Publications
 GUCY2C recombinant protein  >1 publications with GUCA2A and GUCY2C
Pathways associated with GUCA2A recombinant protein
Organs/Tissues associated with GUCA2A recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >1 publications with GUCA2A and Intestine
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