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GUCA2B elisa kit :: Human GUCA2B ELISA Kit

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Catalog # MBS2511524
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

GUCA2B, ELISA Kit

Popular Item
Also Known As

Human GUCA2B (Guanylate Cyclase Activator 2B) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
112
OMIM
601271
3D Structure
ModBase 3D Structure for Q16661
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GUCA2B. No significant cross-reactivity or interference between Human GUCA2B and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GUCA2B and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GUCA2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGUCA2Bpurchase
MBS2511524 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GUCA2B, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GUCA2B. The ELISA analytical biochemical technique of the MBS2511524 kit is based on GUCA2B antibody-GUCA2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GUCA2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GUCA2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GUCA2B elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GUCA2B in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GUCA2B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GUCA2B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GUCA2B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GUCA2B. You can calculate the concentration of Human GUCA2B in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of GUCA2B elisa kit
GUCA2B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2511524. Click to request current manual
NCBI/Uniprot data below describe general gene information for GUCA2B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,069 Da
NCBI Official Full Name
guanylate cyclase activator 2B
NCBI Official Synonym Full Names
guanylate cyclase activator 2B (uroguanylin)
NCBI Official Symbol
GUCA2B  [Similar Products]
NCBI Official Synonym Symbols
UGN; GCAP-II
  [Similar Products]
NCBI Protein Information
guanylate cyclase activator 2B; prepro-uroguanylin
UniProt Protein Name
Guanylate cyclase activator 2B
UniProt Synonym Protein Names
Guanylate cyclase C-activating peptide II; GCAP-II
UniProt Gene Name
GUCA2B  [Similar Products]
UniProt Synonym Gene Names
GCAP-II; UGN  [Similar Products]
UniProt Entry Name
GUC2B_HUMAN
NCBI Summary for GUCA2B
This gene encodes a member of the guanylin family, and is expressed in the stomach and intestine. This protein functions as an endogenous ligand for the guanylate cyclase-C receptor and stimulates an increase in cyclic GMP, a key component of several intracellular signal transduction pathways. It maybe involved in salt and water secretion into the intestinal lumen as well as the renal tubules, and thus regulate electrolyte homeostasis in these tissues. [provided by RefSeq, Oct 2011]
UniProt Comments for GUCA2B
GUCA2B: Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport. Belongs to the guanylin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p34-p33

Molecular Function: calcium sensitive guanylate cyclase activator activity

Biological Process: cGMP biosynthetic process; positive regulation of guanylate cyclase activity; negative regulation of blood pressure; body fluid secretion; excretion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withGUCA2B elisa kit
 Reference Product  PubMed Publications
 GUCY2C elisa kit  >1 publications with GUCA2B and GUCY2C
Pathways associated with GUCA2B elisa kit
Organs/Tissues associated with GUCA2B elisa kit
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >3 publications with GUCA2B and Intestine
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