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GUCY2C elisa kit :: Human guanylate cyclase 2C (heat stable enterotoxin receptor) ELISA Kit

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Catalog # MBS935014
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Typical Testing Data/Standard Curve (for reference only)
Product Name

guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C), ELISA Kit

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Full Product Name

Human Heat-stable enterotoxin receptor, GUCY2C ELISA Kit

Product Synonym Names
Human Heat-stable enterotoxin receptor (GUCY2C) ELISA kit; GUC2C; STAR; guanylate cyclase 2C; guanylyl cyclase C; guanylate cyclase 2C (heat stable enterotoxin receptor)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS935014 Testing Data
3D Structure
ModBase 3D Structure for P70106
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human GUCY2C. No significant cross-reactivity or interference between human GUCY2C and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
25 pg/ml-1600 pg/ml.
Sensitivity
The minimum detectable dose of human GUCY2C is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GUCY2C elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GUCY2Cpurchase
MBS935014 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GUCY2C. The ELISA analytical biochemical technique of the MBS935014 kit is based on GUCY2C antibody-GUCY2C antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GUCY2C antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GUCY2C. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GUCY2C elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GUCY2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GUCY2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GUCY2C is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GUCY2C bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of GUCY2C elisa kit
GUCY2C elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS935014. Click to request current manual
NCBI/Uniprot data below describe general gene information for GUCY2C. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
123,120 Da
NCBI Official Full Name
heat-stable enterotoxin receptor
NCBI Official Symbol
Gucy2c  [Similar Products]
NCBI Official Synonym Symbols
GUC2C
  [Similar Products]
NCBI Protein Information
heat-stable enterotoxin receptor; GC-C; STA receptor; guanylyl cyclase C; intestinal guanylate cyclase
UniProt Protein Name
Heat-stable enterotoxin receptor
UniProt Synonym Protein Names
Guanylyl cyclase C; GC-C; Intestinal guanylate cyclase
UniProt Gene Name
GUCY2C  [Similar Products]
UniProt Synonym Gene Names
GUC2C; STA receptor; GC-C  [Similar Products]
UniProt Entry Name
GUC2C_CAVPO
UniProt Comments for GUCY2C
Function: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

Catalytic activity: GTP = 3',5'-cyclic GMP + diphosphate.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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