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GYPA elisa kit :: Monkey Glycophorin A ELISA Kit

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Catalog # MBS7243562
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Glycophorin A (GYPA), ELISA Kit

Popular Item
Also Known As

Monkey Glycophorin A ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of GPA. No significant cross-reactivity or interference between GPA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPA and all the analogues, therefore, cross reaction may still exist in some cases.
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GYPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGYPApurchase
MBS7243562 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycophorin A (GYPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GYPA. The ELISA analytical biochemical technique of the MBS7243562 kit is based on GYPA antibody-GYPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GYPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GYPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GYPA elisa kit
Intended Uses: This GPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||GPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPA antibody and an GPA-HRP conjugate. The assay sample and buffer are incubated together with GPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPA concentration since GPA from samples and GPA-HRP conjugate compete for the anti-GPA antibody binding site. Since the number of sites is limited, as more sites are occupied by GPA from the sample, fewer sites are left to bind GPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPA concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve of GYPA elisa kit
GYPA elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS7243562. Click to request current manual
NCBI/Uniprot data below describe general gene information for GYPA. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
13,000 Da
NCBI Official Full Name
NCBI Official Synonym Full Names
glycophorin A (MNS blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
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NCBI Protein Information
glycophorin-A; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Erik; glycophorin A, GPA; MN sialoglycoprotein; glycophorin Sta type C; sialoglycoprotein alpha; Mi.V glycoprotein (24 AA); glycophorin A (MN blood group); recombinant glycophorin A-B Miltenberger-DR; erythroid-lineage-specific membrane sialoglycoprotein
UniProt Protein Name
UniProt Synonym Protein Names
MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD_antigen: CD235a
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
NCBI Summary for GYPA
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
UniProt Comments for GYPA
GYPA: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). Belongs to the glycophorin A family.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q31.21

Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: viral receptor activity; identical protein binding; protein homodimerization activity

Biological Process: entry of virus into host cell; cytoskeletal anchoring; regulation of response to osmotic stress

Disease: Malaria, Susceptibility To
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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