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GZMA elisa kit :: Mouse granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) ELISA Kit

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Catalog # MBS704766
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $575 +1 FREE 8GB USB
  96-Strip-Wells  /  $835 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
GZMA elisa kit
Product Name

granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) (GZMA), ELISA Kit

Popular Item
Full Product Name

Mouse granzymes A, Gzms-A ELISA Kit

Product Synonym Names
Mouse granzymes A; Gzms-A ELISA Kit; CTLA3; HFSP; CTL tryptase; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease) ; granzyme A; granzyme A (granzyme 1; cytotoxic T-lymphocyte-associated serine esterase 3)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P11032
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse GZMA. No significant cross-reactivity or interference between mouse GZMA and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Detection Range
6.25 pg/ml -400 pg/mL
Sensitivity
<1.56 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GZMA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGZMApurchase
MBS704766 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) (GZMA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GZMA. The ELISA analytical biochemical technique of the MBS704766 kit is based on GZMA antibody-GZMA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GZMA elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GZMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GZMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GZMA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GZMA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS704766. Click to request current manual
NCBI/Uniprot data below describe general gene information for GZMA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,599 Da
NCBI Official Full Name
granzyme A
NCBI Official Synonym Full Names
granzyme A
NCBI Official Symbol
NCBI Official Synonym Symbols
Hf; SE1; TSP1; Ctla3; TSP-1; Ctla-3; AW494114
  [Similar Products]
NCBI Protein Information
granzyme A; H factor; BLT esterase; fragmentin-1; Hanukah factor; serine esterase 1; t cell-specific serine protease 1; autocrine thymic lymphoma granzyme-like serine protease
UniProt Protein Name
Granzyme A
UniProt Synonym Protein Names
Autocrine thymic lymphoma granzyme-like serine protease; CTLA-3; Fragmentin-1; T cell-specific serine protease 1; TSP-1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Ctla-3; Ctla3; Mtsp-1; TSP-1  [Similar Products]
UniProt Entry Name
GRAA_MOUSE
UniProt Comments for GZMA
Function: Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA

Catalytic activity: Hydrolysis of proteins, including fibronectin, type IV collagen and nucleolin. Preferential cleavage: -Arg-|-Xaa-, -Lys-|-Xaa- >> -Phe-|-Xaa- in small molecule substrates.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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