• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

H-hGH elisa kit :: Growth Hormone, High Sensitivity ELISA Kit

Scan QR to view Datasheet
Catalog # MBS590030
Unit / Price
  96 Wells  /  $440 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Growth Hormone, High Sensitivity (H-hGH), ELISA Kit

Also Known As

Human Growth Hormone, High Sensitivity (H-hGH)

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
217
OMIM
139250
Specificity
This kit exhibits no detectable cross-reaction with LH, hCG, TSH, Prolactin, and FSH.
Samples
Serum
Assay Type
Sandwich
Sensitivity
The minimal detectable concentration of hGH by this assay is estimated to be 0.1 ng/mL.
Intended Uses
This high-sensitivity Human Growth Hormone ELISA Kit is to be used for the in vitro quantitative determination of human growth hormone (hGH) concentrations in serum. This kit is intended FOR LABORATORY RESEARCH USE ONLY, and is not to be used in diagnostic or therapeutic procedures.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of H-hGH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forH-hGHpurchase
MBS590030 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Growth Hormone, High Sensitivity (H-hGH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing H-hGH. The ELISA analytical biochemical technique of the MBS590030 kit is based on H-hGH antibody-H-hGH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect H-hGH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, H-hGH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
H-hGH elisa kit
Principle of the assay: This hGH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been precoated with a monoclonal antibody specific to hGH. Standards or samples are then added to the microtiter plate wells and incubated. After wash all wells, hGH if present, will bind to the antibody pre-coated on the wells. In order to quantitatively determine the amount of hGH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific to hGH is added to each well to "sandwich" the hGH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3', 5,5' Tetramethyl-benzidene) substrate solution is added to each well. This enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain hGH and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm. In order to measure the concentration of hGH in the sample; this Human hGH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus GH concentration (ng/mL). The concentration of hGH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: Human growth hormone (GH) is a 22kDa monomeric protein produced and stored in somatotrophs in the anterior pituitary gland. GH is released from the pituitary into the bloodstream in a pulsatile manner under the regulatory control of hypothalamic somatostatin (SS) and GH-releasing factor (GHRF) [1]. The timing and frequency of GH release appears to be regulated by somatostatin, while the amplitude of GH release is determined by GHRF. A minor fraction (~10%) of GH in circulation exists in a smaller 20 kDa form [2]. GH has profound effects on tissue growth and metabolism, which is thought to be mediated through GH-dependent production of IGF-I and IGF-II, and their associated binding proteins. GH apparently stimulates IGF production after binding to specific cell surface receptors in the liver and, possibly, other tissues. Almost 50% of GH in circulation is bound to a high affinity GH binding protein (GHBP), which represents the extracellular domain of the GH receptor. Deficient GH secretion can occur in a number of clinical conditions [3]. However, evaluation of GH deficiency is complicated by the episodic nature of GH secretion and low circulating levels. A variety of physiologic and pharmacologic stimuli have been used to stimulate pituitary GH release during testing and failure to achieve a normal serum GH level in response to at least 2 GH stimulation or provocative tests is considered to be a diagnostic of GH deficiency [4]. The definition of a normal serum GH response is controversial, although published values generally range from 5 to 10 ng/mL. GH excess (or acromegaly) can be caused either by direct GH hypersecretion or GH excess secondary to GHRF hypersecretion.
Product Categories/Family for H-hGH elisa kit

Typical Testing Data/Standard Curve (for reference only) of H-hGH elisa kit
H-hGH elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS590030. Click to request current manual
NCBI/Uniprot data below describe general gene information for H-hGH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,201 Da
NCBI Official Full Name
growth hormone
NCBI Official Synonym Full Names
growth hormone 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GH; GHN; GH-N; hGH-N; IGHD1B
  [Similar Products]
NCBI Protein Information
somatotropin; pituitary growth hormone
UniProt Protein Name
Somatotropin
UniProt Synonym Protein Names
Growth hormone; GH; GH-N; Growth hormone 1; Pituitary growth hormone
UniProt Gene Name
UniProt Entry Name
SOMA_HUMAN
NCBI Summary for H-hGH
The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]
UniProt Comments for H-hGH
GH: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A); also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH. Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH. Defects in GH1 are the cause of Kowarski syndrome (KWKS); also known as pituitary dwarfism VI. Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2). IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth hormone receptor binding; growth factor activity; prolactin receptor binding; hormone activity; metal ion binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of receptor internalization; positive regulation of JAK-STAT cascade; glucose transport; positive regulation of multicellular organism growth; JAK-STAT cascade; response to estradiol stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Isolated Growth Hormone Deficiency, Type Ia; Isolated Growth Hormone Deficiency, Type Ib; Isolated Growth Hormone Deficiency, Type Ii; Kowarski Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000