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anti-H3K27me1 antibody :: Rabbit H3K27me1 Antibody

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Catalog # MBS126188
Unit / Price
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  0.05 mL  /  $235 +1 FREE 8GB USB
  0.1 mL  /  $350 +1 FREE 8GB USB
  0.2 mL  /  $525 +1 FREE 8GB USB
Product Name

H3K27me1, Antibody

Popular Item
Full Product Name

Histone H3K27me1 Polyclonal Antibody

Product Synonym Names
MonoMethyl-Histone H3-K27: HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602820
3D Structure
ModBase 3D Structure for Q16695
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Other (Wide Range)
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Route
Synthetic Peptide
Immunogen
A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Calculated Molecular Weight
15kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-H3K27me1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-H3K27me1 antibody
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Product Categories/Family for anti-H3K27me1 antibody
Applications Tested/Suitable for anti-H3K27me1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq
Application Notes for anti-H3K27me1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-H3K27me1 antibody
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K27 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
anti-H3K27me1 antibody Western Blot (WB) (WB) image
Dot Blot (DB) of anti-H3K27me1 antibody
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K27 antibody.
anti-H3K27me1 antibody Dot Blot (DB) image
Immunohistochemistry (IHC) of anti-H3K27me1 antibody
Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).
anti-H3K27me1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-H3K27me1 antibody
Immunohistochemistry of paraffin-embedded human thyroid cancer using MonoMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).
anti-H3K27me1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-H3K27me1 antibody
Immunohistochemistry of paraffin-embedded rat lung using MonoMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).
anti-H3K27me1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-H3K27me1 antibody
Immunohistochemistry of paraffin-embedded human kidney cancer using MonoMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).
anti-H3K27me1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-H3K27me1 antibody
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K27 antibody. Blue: DAPI for nuclear staining.
anti-H3K27me1 antibody Immunofluorescence (IF) image
Chromatin Immunoprecipitation (ChIP) of anti-H3K27me1 antibody
Chromatin immunoprecipitation analysis extracts of 293T cells, using MonoMethyl-Histone H3-K27 antibody and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
anti-H3K27me1 antibody Chromatin Immunoprecipitation (ChIP) (IP) image
NCBI/Uniprot data below describe general gene information for H3K27me1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
136
NCBI Official Full Name
Histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3  [Similar Products]
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
  [Similar Products]
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name
Histone H3.1t
UniProt Synonym Protein Names
H3/g
UniProt Gene Name
HIST3H3  [Similar Products]
UniProt Synonym Gene Names
H3FT; H3/t; H3t  [Similar Products]
UniProt Entry Name
H31T_HUMAN
NCBI Summary for H3K27me1
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Comments for H3K27me1
HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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