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anti-H3K27me3 antibody :: Rabbit H3K27me3 Antibody

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Catalog # MBS126239
Unit / Price
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  0.05 mL  /  $235 +1 FREE 8GB USB
  0.1 mL  /  $350 +1 FREE 8GB USB
  0.2 mL  /  $525 +1 FREE 8GB USB
Product Name

H3K27me3, Antibody

Popular Item
Full Product Name

Histone H3K27me3 Polyclonal Antibody

Product Synonym Names
TriMethyl-Histone H3-K27: HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602820
3D Structure
ModBase 3D Structure for Q16695
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Other (Wide Range)
Purity/Purification
Affinity Purification
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Immunogen
A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Calculated Molecular Weight
15kDa
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-H3K27me3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-H3K27me3 antibody
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Product Categories/Family for anti-H3K27me3 antibody
Applications Tested/Suitable for anti-H3K27me3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq
Application Notes for anti-H3K27me3 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:20 - 1:100

Western Blot (WB) of anti-H3K27me3 antibody
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
anti-H3K27me3 antibody Western Blot (WB) (WB) image
Dot Blot (DB) of anti-H3K27me3 antibody
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody.
anti-H3K27me3 antibody Dot Blot (DB) image
Immunohistochemistry (IHC) of anti-H3K27me3 antibody
Immunohistochemistry of paraffin-embedded rat ovary using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100 (40x lens).
anti-H3K27me3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-H3K27me3 antibody
Immunohistochemistry of paraffin-embedded human breast using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100 (40x lens)
anti-H3K27me3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-H3K27me3 antibody
Immunohistochemistry of paraffin-embedded human breast cancer using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100 (40x lens)
anti-H3K27me3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-H3K27me3 antibody
Immunohistochemistry of paraffin-embedded mouse kidney using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100 (40x lens)
anti-H3K27me3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-H3K27me3 antibody
Immunohistochemistry of paraffin-embedded mouse kidney using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100 (40x lens)
anti-H3K27me3 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-H3K27me3 antibody
Immunofluorescence analysis of 293T Cell using TryMethyl-Histone H3-K27 antibody (MBS126239), Blue DAPI for nuclear staining
anti-H3K27me3 antibody Immunofluorescence (IF) image
Immunofluorescence (IF) of anti-H3K27me3 antibody
Immunofluorescence anlysis of Hela cells using TryMethyl-Histone H3-K27 antibody red MBS126239 and BrdU antibody green MBS126158. Blue: DAPI for nuclear staining
anti-H3K27me3 antibody Immunofluorescence (IF) image
Immunofluorescence (IF) of anti-H3K27me3 antibody
Immunofluorescence analysis of Hela cells using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100. Blue : DAPI for nuclear staining
anti-H3K27me3 antibody Immunofluorescence (IF) image
Immunofluorescence (IF) of anti-H3K27me3 antibody
Immunofluorescence analysis of C6 cells using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100. Blue : DAPI for nuclear staining
anti-H3K27me3 antibody Immunofluorescence (IF) image
Testing Data (TD) of anti-H3K27me3 antibody
Chromatin immunoprecipitaion analysis of 293T cekks using TryMethyl-Histone H3-K27 antibody MBS126239 and rabbit IgG. P1 and P2 were located and ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by ratios of the immunoprecipitated DNA to the imput.
anti-H3K27me3 antibody Testing Data (TD) image
NCBI/Uniprot data below describe general gene information for H3K27me3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3  [Similar Products]
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
  [Similar Products]
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name
Histone H3.1t
UniProt Synonym Protein Names
H3/g
UniProt Gene Name
HIST3H3  [Similar Products]
UniProt Synonym Gene Names
H3FT; H3/t; H3t  [Similar Products]
UniProt Entry Name
H31T_HUMAN
NCBI Summary for H3K27me3
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Comments for H3K27me3
HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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