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HAase elisa kit :: Rat Hyaluronidase ELISA Kit

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Catalog # MBS722262
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Hyaluronidase (HAase), ELISA Kit

Also Known As

Rat Hyaluronidase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
604038
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
0.5-10U/L
Sensitivity
1.0U/L
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HAase elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHAasepurchase
MBS722262 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hyaluronidase (HAase) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HAase. The ELISA analytical biochemical technique of the MBS722262 kit is based on HAase antibody-HAase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HAase antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HAase. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HAase elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This HAase ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HAase. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY HAase ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HAase. Standards or samples are then added to the microtiter plate wells and HAase if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HAase present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HAase are added to each well to “sandwich” the HAase immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HAase and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAase concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HAase elisa kit

Typical Testing Data/Standard Curve (for reference only) of HAase elisa kit
HAase elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS722262. Click to request current manual
NCBI/Uniprot data below describe general gene information for HAase. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,865 Da
NCBI Official Full Name
hyaluronidase
NCBI Official Synonym Full Names
hyaluronoglucosaminidase 3
NCBI Official Symbol
HYAL3  [Similar Products]
NCBI Official Synonym Symbols
LUCA3; HYAL-3; LUCA-3
  [Similar Products]
NCBI Protein Information
hyaluronidase-3; lung carcinoma protein 3
UniProt Protein Name
Hyaluronidase-3
UniProt Synonym Protein Names
Hyaluronoglucosaminidase-3; Lung carcinoma protein 3; LuCa-3
Protein Family
UniProt Gene Name
HYAL3  [Similar Products]
UniProt Synonym Gene Names
LUCA3; Hyal-3; LuCa-3  [Similar Products]
UniProt Entry Name
HYAL3_HUMAN
NCBI Summary for HAase
This gene encodes a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]
UniProt Comments for HAase
HYAL3: a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]

Protein type: EC 3.2.1.35; Secreted; Hydrolase; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: lysosome; extracellular region; cytoplasmic vesicle

Molecular Function: viral receptor activity; hyaluronoglucuronidase activity; hyalurononglucosaminidase activity

Biological Process: response to antibiotic; response to virus; cartilage development; carbohydrate metabolic process; inflammatory response; hyaluronan catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withHAase elisa kit
 Reference Product  PubMed Publications
 IFRD2 elisa kit  >1 publications with HAase and IFRD2
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