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HAO1 assay kit :: General Glycolate Oxidase Assay Kit

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Catalog # MBS8243218
Unit / Price
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  100 Assays  /  $385 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Glycolate Oxidase (HAO1), Assay Kit

Popular Item
Full Product Name

Glycolate Oxidase Microplate Assay Kit

Product Synonym Names
EC 1.1.3.15; (S)-2-hydroxy-acid oxidase; Glycolate oxidase; Hydroxy-acid oxidase A; Hydroxy-acid oxidase B; HAO1; GOX; GOX1; HAOX1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS8243218 MSDS
OMIM
605023
Species Reactivity
Detection Range
0.1 mmol/L - 5 mmol/L
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HAO1 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HAO1 assay kit
Background: Glycolate oxidase is a member of the superfamily of the a-hydroxy acid oxidases (HAO), enzymes that are present in both plants and animals. It catalyzes the FMN-mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. The assay is initiated with the enzymatic oxidization of the Glycolic acid by Glycolate oxidase. The enzyme catalysed reaction product Glyoxylic acid react with Phenylhydrazine, glyoxylate phenylhydrazone can be measured at a colorimetric readout at 500 nm.
Applications Tested/Suitable for HAO1 assay kit
Functional Assay

Typical Testing Data/Standard Curve (for reference only) of HAO1 assay kit
HAO1 assay kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS8243218. Click to request current manual
NCBI/Uniprot data below describe general gene information for HAO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,924 Da[Similar Products]
NCBI Official Full Name
glycolate oxidase
NCBI Official Synonym Full Names
hydroxyacid oxidase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GOX; GOX1; HAOX1
  [Similar Products]
NCBI Protein Information
hydroxyacid oxidase 1
UniProt Protein Name
Hydroxyacid oxidase 1
UniProt Synonym Protein Names
Glycolate oxidase; GOX
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GOX1; HAOX1; HAOX1; GOX  [Similar Products]
UniProt Entry Name
HAOX1_HUMAN
NCBI Summary for HAO1
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]
UniProt Comments for HAO1
HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.3.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: peroxisomal matrix; peroxisome

Molecular Function: (S)-2-hydroxy-acid oxidase activity; FMN binding; glycolate oxidase activity; glyoxylate oxidase activity; receptor binding

Biological Process: fatty acid alpha-oxidation; glycolate catabolic process; glyoxylate metabolic process; response to oxidative stress
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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