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HAV IgA elisa kit :: Human HAV IgA ELISA Kit

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Catalog # MBS732285
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

HAV IgA, ELISA Kit

Popular Item
Full Product Name

Human HAV IgA antibody ELISA Kit

Product Synonym Names
Human HAV IgA antibody ELISA Kit; HAV IgA; HAV IgA antibody ELISA Kit (Human)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
Sensitivity: The sensitivity in this assay is 0.1ng/ml

Specificity: This assay has high sensitivity and excellent specificity for detection of ANTI-HAV IGA. No significant cross-reactivity or interference between ANTI-HAV IGA and analogues was observed.
Samples
Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HAV IgA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHAV IgApurchase
MBS732285 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HAV IgA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HAV IgA. The ELISA analytical biochemical technique of the MBS732285 kit is based on HAV IgA antibody-HAV IgA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HAV IgA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HAV IgA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HAV IgA elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This ANTI-HAV IGA ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ANTI-HAV IGA in the sample, this ANTI-HAV IGA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ANTI-HAV IGA concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ANTI-HAV IGA utilizes a multiclonal anti-ANTI-HAV IGA antibody and an ANTI-HAV IGA-HRP conjugate. The assay sample and buffer are incubated together with ANTI-HAV IGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANTI-HAV IGA concentration since ANTI-HAV IGA from samples and ANTI-HAV IGA-HRP conjugate compete for the anti-ANTI-HAV IGA antibody binding site. Since the number of sites is limited, as more sites are occupied by ANTI-HAV IGA from the sample, fewer sites are left to bind ANTI-HAV IGA-HRP conjugate. Standards of known ANTI-HAV IGA concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of ANTI-HAV IGA. The ANTI-HAV IGA concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HAV IgA elisa kit

Typical Testing Data/Standard Curve (for reference only) of HAV IgA elisa kit
HAV IgA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS732285. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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