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HB-EGF elisa kit :: Mouse Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF) ELISA Kit

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Catalog # MBS267401
Unit / Price
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  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF), ELISA Kit

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Full Product Name

Mouse Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
No cross-reaction with other factors.
Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HB-EGF concentration
Assay Type
Detection Range
1000 pg/ml-15.6 pg/ml
5 pg/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HB-EGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HB-EGFpurchase
MBS267401 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HB-EGF. The ELISA analytical biochemical technique of the MBS267401 kit is based on HB-EGF antibody-HB-EGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HB-EGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HB-EGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HB-EGF elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse HB-EGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of HB-EGF elisa kit
HB-EGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS267401. Click to request current manual
NCBI/Uniprot data below describe general gene information for HB-EGF. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Related Accession #
Molecular Weight
22,808 Da
NCBI Official Full Name
heparin-binding epidermal growth factor-like growth factor
NCBI Official Synonym Full Names
heparin-binding EGF-like growth factor
NCBI Official Symbol
Hbegf  [Similar Products]
NCBI Official Synonym Symbols
Dtr; Dts; Hegfl; AW047313
  [Similar Products]
NCBI Protein Information
proheparin-binding EGF-like growth factor; diphtheria toxin receptor; heparin binding epidermal growth factor-like growth factor
UniProt Protein Name
Proheparin-binding EGF-like growth factor
UniProt Gene Name
Hbegf  [Similar Products]
UniProt Synonym Gene Names
Dtr; Hegfl; HB-EGF; HBEGF  [Similar Products]
UniProt Entry Name
UniProt Comments for HB-EGF
HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; positive regulation of protein kinase B signaling cascade; cell migration; negative regulation of elastin biosynthetic process; positive regulation of smooth muscle cell proliferation; blastocyst growth; angiogenesis; positive regulation of cell growth; regulation of heart contraction; positive regulation of cell migration; wound healing, spreading of epidermal cells
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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