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Hepc elisa kit :: Bovine Hepcidin ELISA Kit

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Catalog # MBS734337
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Hepcidin, ELISA Kit

Popular Item
Also Known As

Bovine Hepcidin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 613313
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of HEPC. No significant cross-reactivity or interference between HEPC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HEPC and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
1.0ng/mL
Intended Uses
This HEPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine HEPC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Hepc elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHepcpurchase
MBS734337 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepcidin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hepc. The ELISA analytical biochemical technique of the MBS734337 kit is based on Hepc antibody-Hepc antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hepc antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hepc. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Hepc elisa kit
Principle of the assay: HEPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HEPC antibody and an HEPC-HRP conjugate. The assay sample and buffer are incubated together with HEPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HEPC concentration since HEPC from samples and HEPC-HRP conjugate compete for the anti-HEPC antibody binding site. Since the number of sites is limited, as more sites are occupied by HEPC from the sample, fewer sites are left to bind HEPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards.The HEPC concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Hepc elisa kit

Typical Testing Data/Standard Curve (for reference only) of Hepc elisa kit
Hepc elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS734337. Click to request current manual
NCBI/Uniprot data below describe general gene information for Hepc. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
9,408 Da
NCBI Official Full Name
hepcidin preproprotein
NCBI Official Synonym Full Names
hepcidin antimicrobial peptide
NCBI Official Symbol
NCBI Official Synonym Symbols
HEPC; PLTR; HFE2B; LEAP1
  [Similar Products]
NCBI Protein Information
hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1
UniProt Protein Name
Hepcidin
UniProt Synonym Protein Names
Liver-expressed antimicrobial peptide 1; LEAP-1; Putative liver tumor regressor; PLTR
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
HEPC; LEAP1; UNQ487/PRO1003; LEAP-1; PLTR; Hepc25; Hepc20  [Similar Products]
UniProt Entry Name
HEPC_HUMAN
NCBI Summary for Hepc
The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]
UniProt Comments for Hepc
HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: apical cortex; extracellular region

Molecular Function: hormone activity

Biological Process: cellular iron ion homeostasis; killing of cells of another organism; defense response to bacterium; immune response; defense response to fungus

Disease: Hemochromatosis, Type 2b
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with Hepc elisa kit
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