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HER3 / ErbB3 recombinant protein

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Catalog # MBS2546418
Unit / Price
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  0.01 mg  /  $435 +1 FREE 8GB USB
  0.02 mg  /  $735 +1 FREE 8GB USB
SDS-PAGE
Product Name

HER3 / ErbB3, Recombinant Protein

Popular Item
Full Product Name

Recombinant Human HER3 / ErbB3 Protein (His tag)

Product Synonym Names
HER3, LCCS2, ErbB-3, c-erbB3, erbB3-S, MDA-BF-1, MGC88033, c-erbB-3, p180-ErbB3, p45-sErbB3, p85-sErbB3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
M29366 mRNA
3D Structure
ModBase 3D Structure for P21860
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween80.
Source
Human
Predicted N Terminal
Ser 20
Bio-Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized human ErbB3 at 2 ug/mL (100 ul/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 ug/mL.
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Reconstitution
It is recommended that sterile water (70 ul) be added to the vial to prepare a stock solution of 0.15 mg/mL.
Concentration is measured by UV-Vis.
Preparation and Storage
Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of HER3 / ErbB3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HER3 / ErbB3 recombinant protein
Background: ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Description: A DNA sequence encoding the extracellular domain (Met 1-Thr 643) of human ErbB3 (NP_001973.2) precursor was expressed, with a C-terminal polyhistidine tag.
Application Notes for HER3 / ErbB3 recombinant protein
The recombinant human ErbB3 protein consists of 635 amino acids after the removal of signal peptide and has a calculated molecular mass of 70.2 kDa. As a result of glycosylation, the recombinant ErbB3 migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.

NCBI/Uniprot data below describe general gene information for HER3 / ErbB3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
77,426 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-3 isoform 1
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 3
NCBI Official Symbol
ERBB3  [Similar Products]
NCBI Official Synonym Symbols
HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
  [Similar Products]
NCBI Protein Information
receptor tyrosine-protein kinase erbB-3
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-3
UniProt Synonym Protein Names
Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3
UniProt Gene Name
ERBB3  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for HER3 / ErbB3
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for HER3 / ErbB3
HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: basolateral plasma membrane; extracellular space; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein heterodimerization activity; protein tyrosine kinase activator activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor activity

Biological Process: cranial nerve development; heart development; MAPKKK cascade; negative regulation of cell adhesion; negative regulation of neuron apoptosis; negative regulation of secretion; negative regulation of signal transduction; neuron apoptosis; peripheral nervous system development; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; Schwann cell differentiation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lethal Congenital Contracture Syndrome 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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