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HES1 elisa kit :: Human hairy and enhancer of split 1, (Drosophila) ELISA Kit

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Catalog # MBS910338
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

hairy and enhancer of split 1, (Drosophila) (HES1), ELISA Kit

Popular Item
Full Product Name

Human Transcription factor HES-1, HES1 ELISA Kit

Product Synonym Names
Human Transcription factor HES-1 (HES1) ELISA kit; FLJ20408; HES-1; HHL; HRY; bHLHb39; hairy and enhancer of split 1; hairy homolog; transcription factor HES-1; hairy and enhancer of split 1; (Drosophila)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS910338 Testing Data
OMIM
139605
3D Structure
ModBase 3D Structure for Q14469
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human HES1. No significant cross-reactivity or interference between human HES1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
28 pg/ml -1800 pg/ml.
Sensitivity
The minimum detectable dose of human HES1 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HES1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHES1purchase
MBS910338 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hairy and enhancer of split 1, (Drosophila) (HES1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HES1. The ELISA analytical biochemical technique of the MBS910338 kit is based on HES1 antibody-HES1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HES1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HES1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HES1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HES1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HES1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HES1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HES1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of HES1 elisa kit
HES1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS910338. Click to request current manual
NCBI/Uniprot data below describe general gene information for HES1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,541 Da
NCBI Official Full Name
transcription factor HES-1
NCBI Official Synonym Full Names
hes family bHLH transcription factor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
HHL; HRY; HES-1; bHLHb39
  [Similar Products]
NCBI Protein Information
transcription factor HES-1; hairy homolog; hairy-like protein; class B basic helix-loop-helix protein 39
UniProt Protein Name
Transcription factor HES-1
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 39; bHLHb39; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein; hHL
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
BHLHB39; HL; HRY; bHLHb39; hHL  [Similar Products]
UniProt Entry Name
HES1_HUMAN
NCBI Summary for HES1
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]
UniProt Comments for HES1
HES1: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

Protein type: Cell development/differentiation; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; chaperone binding; histone deacetylase binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; radial glial cell differentiation in the forebrain; hindbrain morphogenesis; midbrain-hindbrain boundary morphogenesis; somatic stem cell maintenance; regulation of fat cell differentiation; regulation of timing of neuron differentiation; cell maturation; positive regulation of JAK-STAT cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of cell proliferation; midbrain development; adenohypophysis development; positive regulation of T cell proliferation; protein complex assembly; trochlear nerve development; positive regulation of mitotic cell cycle, embryonic; oculomotor nerve development; cell adhesion; positive regulation of BMP signaling pathway; positive regulation of Notch signaling pathway; positive regulation of astrocyte differentiation; STAT protein nuclear translocation; positive regulation of DNA binding; nervous system development; cell migration; Notch signaling pathway; smoothened signaling pathway; auditory receptor cell fate determination; thymus development; liver development; endocrine pancreas development; negative regulation of oligodendrocyte differentiation; telencephalon development; lateral inhibition; negative regulation of auditory receptor cell differentiation; neuron morphogenesis during differentiation; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; lung development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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