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Hif-1a recombinant protein

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Catalog # MBS203066
Unit / Price
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  0.1 mg  /  $255 +1 FREE 8GB USB
  0.5 mg  /  $535 +1 FREE 8GB USB
SDS-Page
Product Name

Hif-1a, Recombinant Protein

Popular Item
Full Product Name

Hif-1a, 530-826aa, Human, E Coli

Product Synonym Names
Hypoxia-inducible factor-1a; HIF1A; MOP1; Hif-1 alpha (530-826 aa); HIF-1 alpha; HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein MOP1; Hypoxia-inducible factor 1 alpha; Hypoxia-inducible factor-1a Basic helix loop helix PAS protein MOP1; ARNT interacting protein; bHLHe78; HIF 1A; HIF 1alpha; HIF1 A; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; PASD 8; PASD8
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN
OMIM
U22431 mRNA
3D Structure
ModBase 3D Structure for Q16665
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl pH7.5, 1mMDTT
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of Hif-1a recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Hif-1a recombinant protein
Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1beta subunit and one of three subunits (Hif-1alpha, Hif-2 or Hif-3). The activation of Hif-1 is closely associated with a variety of tumors and oncogenic pathways. Hif-1alpha consists of DNA binding domain (DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Hif-1 alpha (530-826 residues) contains two TAD and inhibitory domain. Recombinant Hif-1 alpha (530-826 residues) was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for Hif-1a recombinant protein
Applications Tested/Suitable for Hif-1a recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for Hif-1a. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
32.8kDa (298aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 1
NCBI Official Synonym Full Names
hypoxia inducible factor 1 alpha subunit
NCBI Official Symbol
HIF1A  [Similar Products]
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
  [Similar Products]
NCBI Protein Information
hypoxia-inducible factor 1-alpha
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Synonym Protein Names
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1
UniProt Gene Name
HIF1A  [Similar Products]
UniProt Synonym Gene Names
bHLHe78  [Similar Products]
NCBI Summary for Hif-1a
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
UniProt Comments for Hif-1a
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Product References and Citations for Hif-1a recombinant protein
Okuyama H. et al., (2006) J. Biol Chem. 281 (22):15554-63; Berra E. et al., (2006) EMBO.7 (1):41-5;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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