• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

HIF-2 elisa kit :: Human Hypoxia-inducible factor 2 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS284375
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $745 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,405 +4 FREE 8GB USB
  10x96-Strip-Wells  /  $6,425 +8 FREE 8GB USB
HIF-2 elisa kit
Product Name

Hypoxia-inducible factor 2 (HIF-2), ELISA Kit

Popular Item
Also Known As

Human Hypoxia-inducible factor 2 (HIF-2) ELISA Kit

Product Synonym Names
ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73; HIF-1alpha-like factor|basic-helix-loop-helix-PAS protein MOP2|hypoxia-inducible factor 2 alpha|member of PAS protein 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
U81984 mRNA
3D Structure
ModBase 3D Structure for Q99814
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human HIF-2. No significant cross-reactivity or interference between Human HIF-2 and analogues was observed.
Samples
serum, plasma, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/mL - 2000 pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HIF-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHIF-2purchase
MBS284375 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hypoxia-inducible factor 2 (HIF-2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HIF-2. The ELISA analytical biochemical technique of the MBS284375 kit is based on HIF-2 antibody-HIF-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HIF-2 elisa kit
Intended Uses: For the quantitative detection of Human Hypoxia-inducible factor 2 (HIF-2) concentration in serum, plasma, cell lysates and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HIF-2 in Human serum, plasma, cell lysates. An antibody specific for HIF-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS284375. Click to request current manual
NCBI/Uniprot data below describe general gene information for HIF-2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
96,459 Da
NCBI Official Full Name
endothelial PAS domain-containing protein 1
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
EPAS1  [Similar Products]
NCBI Official Synonym Symbols
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
  [Similar Products]
NCBI Protein Information
endothelial PAS domain-containing protein 1
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Synonym Protein Names
Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
UniProt Gene Name
EPAS1  [Similar Products]
UniProt Synonym Gene Names
BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha  [Similar Products]
NCBI Summary for HIF-2
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
UniProt Comments for HIF-2
HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: cytosol; nuclear speck; nucleoplasm; nucleus; transcription factor complex

Molecular Function: DNA binding; histone acetyltransferase binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor binding

Biological Process: angiogenesis; blood vessel remodeling; cell maturation; cellular response to hypoxia; embryonic placenta development; erythrocyte differentiation; iron ion homeostasis; lung development; mitochondrion organization; myoblast cell fate commitment; norepinephrine metabolic process; positive regulation of transcription from RNA polymerase II promoter; post-translational protein modification; protein ubiquitination; regulation of heart rate; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; response to hypoxia; signal transduction; somatic stem cell maintenance; surfactant homeostasis; transcription from RNA polymerase II promoter; visual perception

Disease: Erythrocytosis, Familial, 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000