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HIF1AN recombinant protein

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Catalog # MBS203207
Unit / Price
  0.1 mg  /  $255 +1 FREE 8GB USB
  0.5 mg  /  $535 +1 FREE 8GB USB
SDS-Page
Product Name

HIF1AN, Recombinant Protein

Also Known As

HIF1AN, 1-349aa, Human, E Coli

Product Synonym Names
Hypoxia-inducible factor 1 alpha subunit inhibitor; Hypoxia-inducible factor asparagine hydroxylase; Factor inhibiting HIF-1; FIH1; Hypoxia-inducible factor 1; alpha subunit inhibitor Hypoxia inducible factor asparagine hydroxylase; Factor inhibiting HIF1; FIH 1; FLJ20615; FLJ22027; Hypoxia inducible factor 1 alpha inhibitor; Hypoxia inducible factor 1 alpha subunit inhibitor; Peptide aspartate beta dioxygenase; DKFZp762F1811; Hypoxia-Inducible Factor-1 Alpha Inhibitor
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
349
Sequence
MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGYKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE RFPNFQNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN
OMIM
AF395830 mRNA
3D Structure
ModBase 3D Structure for Q9NWT6
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0)
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of HIF1AN recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HIF1AN recombinant protein
Description: Hypoxia-inducible factor 1, alpha subunit inhibitor (HIF1AN), a member of the Fe2+ and 2-oxoglutarate-dependent dioxygenase superfamily, is protein that hydoxylates a specific asparagine residue (Asn-803) within the HIF1alpha C-terminal transactivation domain. In normoxia, the HIF1AN-mediated hydroxylation of the HIF1alpha transactivation domain results in blockage of the HIF1alpha-p300/CBP interaction and represses transcriptional activity of HIF1alpha-targeted genes. Recombinant human HIF1AN protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for HIF1AN recombinant protein
Applications Tested/Suitable for HIF1AN recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for HIF1AN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40.2 kDa (349 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
hypoxia-inducible factor 1-alpha inhibitor
NCBI Official Synonym Full Names
hypoxia inducible factor 1 alpha subunit inhibitor
NCBI Official Symbol
HIF1AN  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
hypoxia-inducible factor 1-alpha inhibitor
UniProt Protein Name
Hypoxia-inducible factor 1-alpha inhibitor
UniProt Synonym Protein Names
Factor inhibiting HIF-1; FIH-1; Hypoxia-inducible factor asparagine hydroxylase
UniProt Gene Name
HIF1AN  [Similar Products]
UniProt Synonym Gene Names
FIH1; FIH-1  [Similar Products]
UniProt Comments for HIF1AN
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Product References and Citations for HIF1AN recombinant protein
Fukuba H., et al. (2008) Neurosci Lett. 433 (3):209-14.; Li J., et al. (2007) Mol Cell Biol. 27 (20):7345-53.;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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