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HIF2a recombinant protein :: Hypoxia Inducible Factor 2 Alpha (HIF2a) Recombinant Protein

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Catalog # MBS2009225 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $265 +1 FREE 8GB USB
  0.1 mg  /  $415 +1 FREE 8GB USB
  0.2 mg  /  $510 +1 FREE 8GB USB
  0.5 mg  /  $990 +1 FREE 8GB USB
  1 mg  /  $1,465 +1 FREE 8GB USB
HIF2a recombinant protein
Product Name

Hypoxia Inducible Factor 2 Alpha (HIF2a), Recombinant Protein

Also Known As

Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
874
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-DAARCR RSKE TEVFYELAHE LPLPHSVSSH LDKASIMRLA ISFLRTHKLL SSVCSENESE AEADQQMDNL YLKALEGFIA VVTQDGDMIF LSENISKFMG LTQVELTGHS IFDFTHPCDH EEIRENLTLK NGSGFGKKSK DVSTERDFFM RMKCTVTNRG RTVNLKSATW KVLHCTGQVR VYNNCPPHSS LCGSKEPLLS CLIIMCEPIQ HPSHMDIPLD SKTFLSRHSM DMKFTYCDDR ILELIGYHPE ELLGRSAYEF YHALDSENMT KSHQNLCTKG QVVSGQYRML AKHGGYVWLE TQGTVIYNPR NLQPQC
Chromosome Location
Chromosome: 17; NC_000083.6 (86753864..86833410). Location: 17 E4; 17
3D Structure
ModBase 3D Structure for P97481
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Asp21~Cys336 (Accession # P97481) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of HIF2a recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HIF2a recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for HIF2a recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
NCBI/Uniprot data below describe general gene information for HIF2a. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37.5kDa
NCBI Official Full Name
endothelial PAS domain-containing protein 1
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
Epas1  [Similar Products]
NCBI Official Synonym Symbols
HLF; HRF; MOP2; HIF2A; bHLHe73; HIF-2alpha
  [Similar Products]
NCBI Protein Information
endothelial PAS domain-containing protein 1; mHLF; EPAS-1; HIF2-alpha; HIF-2-alpha; Hif like protein; HIF-related factor; HIF1alpha-like factor; HIF1 alpha-like factor; HIF-1-alpha-like factor; HLF (HIF1alpha-like factor); hypoxia-inducible factor 2-alpha; hypoxia inducible transcription factor 2alpha
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Synonym Protein Names
HIF-1-alpha-like factor; HLF; mHLF; HIF-related factor; HRF; Hypoxia-inducible factor 2-alpha
UniProt Gene Name
Epas1  [Similar Products]
UniProt Synonym Gene Names
Hif2a; EPAS-1; HLF; mHLF; HRF; HIF-2-alpha; HIF2-alpha  [Similar Products]
UniProt Entry Name
EPAS1_MOUSE
UniProt Comments for HIF2a
HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Protein type: Transcription factor; DNA-binding

Cellular Component: transcription factor complex; cytoplasm; nuclear speck; nucleus

Molecular Function: protein dimerization activity; histone acetyltransferase binding; signal transducer activity; protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; myoblast cell fate commitment; embryonic placenta development; transcription, DNA-dependent; regulation of heart rate; multicellular organismal development; cell maturation; norepinephrine metabolic process; signal transduction; iron ion homeostasis; regulation of transcription, DNA-dependent; visual perception; response to hypoxia; erythrocyte differentiation; blood vessel remodeling; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; cell differentiation; surfactant homeostasis; lung development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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