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HIF3A elisa kit :: Human HIF3A (Hypoxia-inducible factor 3-alpha) ELISA Kit

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Catalog # MBS7606705
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

HIF3A (Hypoxia-inducible factor 3-alpha), ELISA Kit

Popular Item
Also Known As

Human HIF3A (Hypoxia-inducible factor 3-alpha) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS7606705 COA
Sequence Length
664
3D Structure
ModBase 3D Structure for Q0VBL6
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of HIF3A . No significant cross-reactivity or interference between HIF3A and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Sandwich
Detection Range
78-5000pg/ml
Sensitivity
<46.9pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HIF3A were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HIF3A were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HIF3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHIF3Apurchase
MBS7606705 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HIF3A (Hypoxia-inducible factor 3-alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HIF3A. The ELISA analytical biochemical technique of the MBS7606705 kit is based on HIF3A antibody-HIF3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HIF3A elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HIF3A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HIF3A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HIF3A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HIF3A can be calculated.

Typical Testing Data/Standard Curve (for reference only) of HIF3A elisa kit
HIF3A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7606705. Click to request current manual
NCBI/Uniprot data below describe general gene information for HIF3A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
73,122 Da
NCBI Official Full Name
hypoxia-inducible factor 3-alpha isoform 1
NCBI Official Synonym Full Names
hypoxia inducible factor 3, alpha subunit
NCBI Official Symbol
Hif3a  [Similar Products]
NCBI Official Synonym Symbols
Ipas; MOP7; NEPAS; bHLHe17
  [Similar Products]
NCBI Protein Information
hypoxia-inducible factor 3-alpha
UniProt Protein Name
Hypoxia-inducible factor 3-alpha
UniProt Synonym Protein Names
Basic-helix-loop-helix-PAS protein MOP7; HIF3-alpha-1; Inhibitory PAS domain protein
Protein Family
UniProt Gene Name
Hif3a  [Similar Products]
UniProt Synonym Gene Names
UniProt Comments for HIF3A
HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 7|7 A2

Cellular Component: cytoplasm; cytosol; mitochondrion; nuclear speck; nucleoplasm; nucleus; plasma membrane

Molecular Function: DNA binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; response to hypoxia; transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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