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HL elisa kit :: Mouse Hepatic lipase (HL) ELISA Kit

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Catalog # MBS262817
Unit / Price
  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Hepatic lipase (HL), ELISA Kit

Popular Item
Also Known As

Mouse Hepatic lipase (HL) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
499
OMIM
614025
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HL concentration
Assay Type
Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
0.06 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHLpurchase
MBS262817 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepatic lipase (HL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HL. The ELISA analytical biochemical technique of the MBS262817 kit is based on HL antibody-HL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HL elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse HL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of HL elisa kit
HL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS262817. Click to request current manual
NCBI/Uniprot data below describe general gene information for HL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,914 Da
NCBI Official Full Name
hepatic triacylglycerol lipase
NCBI Official Synonym Full Names
lipase, hepatic
NCBI Official Symbol
NCBI Official Synonym Symbols
HL; HTGL; LIPH; HDLCQ12
  [Similar Products]
NCBI Protein Information
hepatic triacylglycerol lipase; hepatic lipase; lipase member C
UniProt Protein Name
Hepatic triacylglycerol lipase
UniProt Synonym Protein Names
Lipase member C
UniProt Gene Name
UniProt Synonym Gene Names
HTGL; HL; Hepatic lipase  [Similar Products]
UniProt Entry Name
LIPC_HUMAN
NCBI Summary for HL
LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]
UniProt Comments for HL
LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Phospholipase; Secreted, signal peptide; Secreted; Lipid Metabolism - glycerolipid; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 15q21-q23

Cellular Component: extracellular space

Molecular Function: heparin binding; triacylglycerol lipase activity; low-density lipoprotein binding; apolipoprotein binding; phospholipase activity

Biological Process: cholesterol metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol catabolic process; fatty acid biosynthetic process

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12; Hepatic Lipase Deficiency; Diabetes Mellitus, Noninsulin-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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