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HLCS elisa kit :: Mouse Biotin--Protein Ligase (HLCS) ELISA Kit

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Catalog # MBS9395668
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HLCS elisa kit
Product Name

Biotin--Protein Ligase (HLCS), ELISA Kit

Full Product Name

Mouse Biotin--Protein Ligase (HLCS) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
D23672 mRNA
3D Structure
ModBase 3D Structure for P50747
Species Reactivity
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HLCS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HLCSpurchase
MBS9395668 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Biotin--Protein Ligase (HLCS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HLCS. The ELISA analytical biochemical technique of the MBS9395668 kit is based on HLCS antibody-HLCS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HLCS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HLCS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for HLCS. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
80,760 Da
NCBI Official Full Name
biotin--protein ligase
NCBI Official Synonym Full Names
holocarboxylase synthetase
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
biotin--protein ligase
UniProt Protein Name
Biotin--protein ligase
UniProt Synonym Protein Names
Biotin apo-protein ligaseIncluding the following 4 domains:Biotin--[methylmalonyl-CoA-carboxytransferase] ligase (EC:[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (EC: name(s):Holocarboxylase synthetase; HCS
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for HLCS
This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011]
UniProt Comments for HLCS
HLCS: Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl- CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase. Defects in HLCS are the cause of holocarboxylase synthetase deficiency (HLCS deficiency); also known as biotin-responsive multiple carboxylase deficiency. HLCS deficiency is a neonatal form of multiple carboxylase deficiency, an autosomal recessive disorder characterized by metabolic ketoacidosis, hyperammonemia, excretion of abnormal organic acid metabolites and dermatitis. Clinical and biochemical symptoms improve dramatically with administration of biotin. Belongs to the biotin--protein ligase family.

Protein type: Cofactor and Vitamin Metabolism - biotin; EC; EC; EC; EC; Ligase; Mitochondrial

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: chromatin; cytoplasm; cytosol; nuclear lamina; nuclear matrix

Molecular Function: biotin binding; biotin-[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase activity; biotin-protein ligase activity; enzyme binding; protein binding

Biological Process: biotin metabolic process; cell proliferation; histone modification; protein amino acid biotinylation

Disease: Holocarboxylase Synthetase Deficiency
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with HLCS elisa kit
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