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HMG-CoA elisa kit :: Canine 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit

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Catalog # MBS744253
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

3-Hydroxy-3-methylglutaryl CoA reductase (HMG-CoA), ELISA Kit

Also Known As

Canine 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ug/mL
Sensitivity
0.1ug/mL
Intended Uses
This HMG-CoA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine HMG-CoA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HMG-CoA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHMG-CoApurchase
MBS744253 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3-Hydroxy-3-methylglutaryl CoA reductase (HMG-CoA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HMG-CoA. The ELISA analytical biochemical technique of the MBS744253 kit is based on HMG-CoA antibody-HMG-CoA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HMG-CoA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HMG-CoA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HMG-CoA elisa kit
Principle of the Assay: HMG-CoA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HMG-CoA antibody and an HMG-CoA-HRP conjugate. The assay sample and buffer are incubated together with HMG-CoA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMG-CoA concentration since HMG-CoA from samples and HMG-CoA-HRP conjugate compete for the anti-HMG-CoA antibody binding site. Since the number of sites is limited, as more sites are occupied by HMG-CoA from the sample, fewer sites are left to bind HMG-CoA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMG-CoA concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HMG-CoA elisa kit

Typical Testing Data/Standard Curve (for reference only) of HMG-CoA elisa kit
HMG-CoA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS744253. Click to request current manual
NCBI/Uniprot data below describe general gene information for HMG-CoA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
97,040 Da
NCBI Official Full Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase
NCBI Official Synonym Full Names
3-hydroxy-3-methylglutaryl-Coenzyme A reductase
NCBI Official Symbol
Hmgcr  [Similar Products]
NCBI Official Synonym Symbols
Red; HMG-CoAR
  [Similar Products]
NCBI Protein Information
3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase; 3-hydroxy-3-methylglutaryl-CoA reductase
UniProt Protein Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase
UniProt Gene Name
Hmgcr  [Similar Products]
UniProt Synonym Gene Names
HMG-CoA reductase  [Similar Products]
UniProt Entry Name
HMDH_MOUSE
UniProt Comments for HMG-CoA
HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; EC 1.1.1.34; Endoplasmic reticulum; Oxidoreductase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Membrane protein, multi-pass

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane

Molecular Function: hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein homodimerization activity; protein phosphatase 2A binding; hydroxymethylglutaryl-CoA reductase activity; oxidoreductase activity; coenzyme binding; NADP binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: steroid metabolic process; negative regulation of MAP kinase activity; cholesterol metabolic process; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; embryonic development; ubiquinone metabolic process; negative regulation of vasodilation; sterol biosynthetic process; positive regulation of cell proliferation; visual learning; lipid metabolic process; steroid biosynthetic process; protein tetramerization; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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