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HMGB-1 elisa kit :: Rat High Mobility Group Protein B1, HMGB-1 ELISA Kit

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Catalog # MBS160240
Unit / Price
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  48-Strip-Wells  /  $280 +1 FREE 8GB USB
  96-Strip-Wells  /  $445 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,830 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,580 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

High Mobility Group Protein B1, HMGB-1, ELISA Kit

Popular Item
Full Product Name

Rat High Mobility Group Protein B1, HMGB-1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q08IE6
Species Reactivity
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.1ng/ml - 30ng/ml
Sensitivity
0.051ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HMGB-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HMGB-1purchase
MBS160240 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the High Mobility Group Protein B1, HMGB-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HMGB-1. The ELISA analytical biochemical technique of the MBS160240 kit is based on HMGB-1 antibody-HMGB-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HMGB-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HMGB-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HMGB-1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat High Mobility Group Protein B1 (also known as HMGB-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HMGB-1 antibody. HMGB-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HMGB-1 Antibody is added and binds to HMGB-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HMGB-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HMGB-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Typical Testing Data/Standard Curve (for reference only) of HMGB-1 elisa kit
HMGB-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS160240. Click to request current manual
NCBI/Uniprot data below describe general gene information for HMGB-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
24,894 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Symbol
HMGB1  [Similar Products]
NCBI Official Synonym Symbols
HMG-1
  [Similar Products]
NCBI Protein Information
high mobility group protein B1
UniProt Protein Name
High mobility group protein B1
UniProt Synonym Protein Names
High mobility group protein 1; HMG-1
UniProt Gene Name
HMGB1  [Similar Products]
UniProt Synonym Gene Names
HMG1; HMG-1  [Similar Products]
UniProt Comments for HMGB-1
Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance. Has proangiogenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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