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hnRNP A2 elisa kit :: Rat Heterogeneous nuclear ribonucleoprotein A2 ELISA Kit

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Catalog # MBS721109
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2), ELISA Kit

Also Known As

Rat Heterogeneous nuclear ribonucleoprotein A2 ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 615422
Species Reactivity
Samples
Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of hnRNP A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forhnRNP A2purchase
MBS721109 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing hnRNP A2. The ELISA analytical biochemical technique of the MBS721109 kit is based on hnRNP A2 antibody-hnRNP A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect hnRNP A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, hnRNP A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
hnRNP A2 elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This HNRNP/RA33 ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of HNRNP/RA33 in the sample, this HNRNP/RA33 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus HNRNP/RA33 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of HNRNP/RA33 utilizes a multiclonal anti-HNRNP/RA33 antibody and an HNRNP/RA33-HRP conjugate. The assay sample and buffer are incubated together with HNRNP/RA33-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HNRNP/RA33 concentration since HNRNP/RA33 from samples and HNRNP/RA33-HRP conjugate compete for the anti-HNRNP/RA33 antibody binding site. Since the number of sites is limited, as more sites are occupied by HNRNP/RA33 from the sample, fewer sites are left to bind HNRNP/RA33-HRP conjugate. Standards of known HNRNP/RA33 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of HNRNP/RA33. The HNRNP/RA33 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of hnRNP A2 elisa kit
hnRNP A2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS721109. Click to request current manual
NCBI/Uniprot data below describe general gene information for hnRNP A2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36,006 Da
NCBI Official Full Name
hnRNP A2 protein
NCBI Official Synonym Full Names
heterogeneous nuclear ribonucleoprotein A2/B1
NCBI Official Symbol
HNRNPA2B1  [Similar Products]
NCBI Official Synonym Symbols
RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; IBMPFD2; HNRPA2B1
  [Similar Products]
NCBI Protein Information
heterogeneous nuclear ribonucleoproteins A2/B1; hnRNP A2 / hnRNP B1; nuclear ribonucleoprotein particle A2 protein
UniProt Protein Name
Heterogeneous nuclear ribonucleoproteins A2/B1
UniProt Gene Name
HNRNPA2B1  [Similar Products]
UniProt Synonym Gene Names
HNRPA2B1; hnRNP A2/B1  [Similar Products]
UniProt Entry Name
ROA2_HUMAN
NCBI Summary for hnRNP A2
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for hnRNP A2
hnRNP A2/B1: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: nucleoplasm; spliceosome; membrane; cytoplasm; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; single-stranded telomeric DNA binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; negative regulation of transcription from RNA polymerase II promoter; mRNA processing; RNA transport

Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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