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HO1 elisa kit :: Monkey Heme Oxygenase 1, Decycling ELISA Kit

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Catalog # MBS740175
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Heme Oxygenase 1, Decycling (HO1), ELISA Kit

Popular Item
Full Product Name

Monkey Heme Oxygenase 1, Decycling ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
This assay has high sensitivity and excellent specificity for detection of HO-1. No significant cross-reactivity or interference between HO-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HO-1 and all the analogues, therefore, cross reaction may still exist in some cases.
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Detection Range
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HO1purchase
MBS740175 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heme Oxygenase 1, Decycling (HO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HO1. The ELISA analytical biochemical technique of the MBS740175 kit is based on HO1 antibody-HO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HO1 elisa kit
Intended Uses: This HO-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey HO-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: HO-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HO-1 antibody and an HO-1-HRP conjugate. The assay sample and buffer are incubated together with HO-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HO-1 concentration since HO-1 from samples and HO-1-HRP conjugate compete for the anti-HO-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HO-1 from the sample, fewer sites are left to bind HO-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HO-1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HO1 elisa kit

Typical Standard Curve/Testing Data of HO1 elisa kit
HO1 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS740175. Click to request current manual
NCBI/Uniprot data below describe general gene information for HO1. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,006 Da
NCBI Official Full Name
heme oxygenase 1
NCBI Official Synonym Full Names
heme oxygenase (decycling) 1
NCBI Official Symbol
Hmox1  [Similar Products]
NCBI Official Synonym Symbols
Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
  [Similar Products]
NCBI Protein Information
heme oxygenase 1; heme oxygenase (decyclizing) 1
UniProt Protein Name
Heme oxygenase 1
UniProt Synonym Protein Names
UniProt Gene Name
Hmox1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
NCBI Summary for HO1
catalyzes the oxidative cleavage of heme to biliverdin [RGD, Feb 2006]
UniProt Comments for HO1
HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family.

Protein type: EC; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; perinuclear region of cytoplasm; plasma membrane; nucleolus; caveola; cytosol; nucleus

Molecular Function: signal transducer activity; protein binding; enzyme binding; protein homodimerization activity; metal ion binding; phospholipase D activity; heme binding; oxidoreductase activity; heme oxygenase (decyclizing) activity

Biological Process: response to nicotine; cell death; negative regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; positive regulation of smooth muscle cell proliferation; heme metabolic process; erythrocyte homeostasis; negative regulation of cell proliferation; regulation of transcription factor activity; heme catabolic process; small GTPase mediated signal transduction; regulation of blood pressure; phospholipid metabolic process; negative regulation of mast cell cytokine production; angiogenesis; negative regulation of neuron apoptosis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; healing during inflammatory response; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; negative regulation of DNA binding; iron ion homeostasis; positive regulation of angiogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; response to hypoxia; response to oxidative stress
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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