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HP elisa kit :: Haptoglobin ELISA Kit

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Catalog # MBS564090
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  1x96 Wells  /  $480 +1 FREE 8GB USB
HP elisa kit
Product Name

Haptoglobin (HP), ELISA Kit

Popular Item
Full Product Name

Pig Haptoglobin ELISA Kit

Product Synonym Names
Pig Haptoglobin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Chromosome Location
chromosome: 16; Location: 16q22.1
OMIM
140100
3D Structure
ModBase 3D Structure for P00738
Host
Pig
Samples
Immunoperoxidase Assay for Determination of Haptoglobin in Pig Samples
Assay Type
Sandwich
Intended Uses
The Haptoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in biological fluid of Pigs.
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HPpurchase
MBS564090 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pig Haptoglobin (HP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HP. The ELISA analytical biochemical technique of the MBS564090 kit is based on HP antibody-HP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HP elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Haptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in Human by Tillet and Frances in 19301. Haptoglobin (Hp) is a heterogeneous plasma protein mostly synthesized by the liver. The haptoglobin monomer consist of two heavy chains, beta chains (40 kD) and two light chains, alpha chains, alpha 1 (9 kD) and alpha 2 (16 kD) that are linked disulfide bonds. The three major haptoglobin types are; Hp1-1 which is monomeric (98kD), Hp1-2 is polymeric at about 200 kD, and Hp2-2 at about 400 kD. The levels in serum rise quickly following acute tissue damage within 24 to 48 hours and also fall very rapidly once the stimulus is removed. In fact, Hp level are decreased in hemolytic anemia. Hp has a high affinity for hemoglobin (Hb) and its function appears to be to prevent loss of Hb in urine which would lead to loss of iron. Investigations over the past few years have shown that quantification of Hp in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in Human, but in companion animals and farm herds as well2.
Product Categories/Family for HP elisa kit
NCBI/Uniprot data below describe general gene information for HP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,205 Da[Similar Products]
NCBI Official Full Name
haptoglobin
NCBI Official Synonym Full Names
haptoglobin
NCBI Official Symbol
NCBI Official Synonym Symbols
BP; HPA1S; HP2ALPHA2; MGC111141; HP
  [Similar Products]
NCBI Protein Information
haptoglobin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide
UniProt Protein Name
Haptoglobin
Protein Family
UniProt Gene Name
UniProt Entry Name
HPT_HUMAN
NCBI Summary for HP
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for HP
HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response

Disease: Anhaptoglobinemia
Research Articles on HP
1. depressed plasma concentrations in women with preeclampsia
2. Specific Hp polymorphisms may be associated with cGVHD development and warrant further investigation.
3. the influences of the haptoglobin, MnSOD, CAT, GPX1, ACE, glutathione S-transferases M1 (GSTM1) and T1 (GSTT1) genes' polymorphisms on the oxidative stress and damage suffered by human runners was studied
4. The results extend previous observations that Hp2-2 is associated with clinical cardiovascular disease in diabetes.
5. Haptoglobin may be a potential useful biomarker for diagnosis or a medicine target for treatment of neuromyelitis optica.
6. Results suggest an association between haptoglobin (Hp) genotypes and the severity of heart complications in Chagas' disease.
7. Our data show that Haptoglobin is a novel monocyte chemoattractant and that its chemotactic potential is mediated, at least in part. by its interaction with CCR2.
8. Our results have identified, for the first time, unique patterns of haptoglobin mRNA expression and protein localization in the stromal and glandular epithelial cells of endometrioid adenocarcinoma of the uterus.
9. Genetic polymorphisms of SLC2A2 and HP is associated with serum cholesterol levels.
10. analysis of the N-glycosylation status of beta-haptoglobin in sera of patients with prostate cancer vs. benign prostate diseases
11. Results identified haptoglobin alpha-1, apolipoprotein C-I and apolipoprotein C-III as candidate biomarkers in PTC.
12. we have now identified human zonulin as the precursor for haptoglobin-2 (pre-HP2).
13. We did not find any association between HP genotypes and type 2 diabetes or the anthropometric and clinical features examined in the population studied.
14. under conditions of severe inflammation and oxidative stress, peroxidase activity of Hb-Hp covalent aggregates may cause macrophage dysfunction and microvascular vasoconstriction
15. Haptoglobin genotype is closely related to recurrence rate inpatients with HNSCC.
16. haptoglobin and serum amyloid A may have roles in atherothrombotic ischemic stroke
17. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
18. Meta-analysis of gene-disease association. (HuGE Navigator)
19. These results indicate that haptoglobin (Hp)improves endothelial progenitor cells (EPCs) function in neovasculogenesis, which suggests that ex vivo modification of EPCs with the Hp gene can be applied to the treatment of vascular damage.
20. HDL can become proinflammatory via the hemoglobin x Hp pathway in mice and humans
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with HP elisa kit
Organs/Tissues associated with HP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >888 publications with HP and Liver
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