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HPSE elisa kit :: Mouse heparanase ELISA Kit

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Catalog # MBS906653
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Typical Testing Data/Standard Curve (for reference only)
Product Name

heparanase (HPSE), ELISA Kit

Popular Item
Full Product Name

Mouse Heparanase, HPSE ELISA Kit

Product Synonym Names
Mouse Heparanase (HPSE) ELISA kit; HPA; HPR1; HPSE1; HSE1; heparanase exon 9&10 deletion; heparanase exon10-deletion; heparanase-1; heparanase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q6YGZ1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse HPSE. No significant cross-reactivity or interference between mouse HPSE and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
31.25 mU/ml -2000 mU/ml.
Sensitivity
The minimum detectable dose of mouse HPSE is typically less than 7.8 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HPSE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HPSEpurchase
MBS906653 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heparanase (HPSE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HPSE. The ELISA analytical biochemical technique of the MBS906653 kit is based on HPSE antibody-HPSE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HPSE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HPSE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HPSE elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HPSE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HPSE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HPSE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HPSE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of HPSE elisa kit
HPSE elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for HPSE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60,066 Da
NCBI Official Full Name
heparanase
NCBI Official Synonym Full Names
heparanase
NCBI Official Symbol
NCBI Official Synonym Symbols
Hpa; HSE1; Hpr1
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NCBI Protein Information
heparanase; endo-glucoronidase; endoglycosidase heparanase
UniProt Protein Name
Heparanase
UniProt Synonym Protein Names
Endo-glucoronidaseCleaved into the following 2 chains:Heparanase 8 kDa subunit; Heparanase 50 kDa subunit
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
HPSE_MOUSE
UniProt Comments for HPSE
HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family.

Protein type: Secreted, signal peptide; EC 3.2.1.166; Extracellular matrix; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; Secreted

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; intracellular membrane-bound organelle; membrane; lysosome; extracellular region; nucleus; lipid raft

Molecular Function: protein dimerization activity; syndecan binding; hydrolase activity; heparanase activity; hydrolase activity, acting on glycosyl bonds

Biological Process: positive regulation of hair follicle development; positive regulation of protein kinase B signaling cascade; positive regulation of blood coagulation; heparan sulfate proteoglycan catabolic process; wound healing; cell-matrix adhesion; carbohydrate metabolic process; positive regulation of osteoblast proliferation; regulation of hair follicle development; angiogenesis involved in wound healing; cell adhesion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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