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Hpt elisa kit :: Goat Haptoglobin (Hpt) ELISA Kit

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Catalog # MBS267429
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Haptoglobin (Hpt), ELISA Kit

Popular Item
Full Product Name

Goat Haptoglobin (Hpt) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 614081
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Goat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Hpt concentration
Assay Type
Sandwich
Detection Range
20 ?g/ml-0.312 ?g/ml
Sensitivity
0.06 ?g/ml.
Intra-assay Precision
? 8%
Inter-assay Precision
? 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Hpt elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Hptpurchase
MBS267429 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Haptoglobin (Hpt) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hpt. The ELISA analytical biochemical technique of the MBS267429 kit is based on Hpt antibody-Hpt antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hpt antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hpt. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Haptoglobin binds hemoglobin. The protein concentration in serum and plasma are frequently used biomarkers for hemolytic anemia and during stages of human pregnancy. Haptoglobin HPT detection can be used as a small molecule screen readout to determine assay precision. Cell or molecular biology experiments benefit from our available HP ELISA kit to quantitate your samples. With all required antibodies, antibody bound plate, standard curve reagent, sample diluent solution and test revealing reagents, you can be assured our ELISA kits perform to exacting ng ml ranges and outstanding intra assay variability. Our secondary antibodies are robust and compare to ICC IHC IHC results. Besides goat, we offer ELISA kit rat HPT and drosophila ELISA kit haptoglobin with similar application details and results. View our complete catalog for all primary antibodies secondary antibodies and a complete comparison list. Order haptoglobin HPT HP kits today, experiment tomorrow.
Related Product Information for
Hpt elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat Hpt monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of Hpt elisa kit
Hpt elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for Hpt. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,452 Da
NCBI Official Full Name
haptoglobin
NCBI Official Synonym Full Names
haptoglobin
NCBI Official Symbol
NCBI Official Synonym Symbols
BP; HPA1S; HP2ALPHA2
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NCBI Protein Information
haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide
UniProt Protein Name
Haptoglobin
UniProt Synonym Protein Names
ZonulinCleaved into the following 2 chains:Haptoglobin alpha chain; Haptoglobin beta chain
UniProt Gene Name
UniProt Entry Name
HPT_HUMAN
NCBI Summary for Hpt
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for Hpt
HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; metabolic process; immune system process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response

Disease: Anhaptoglobinemia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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