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HPX elisa kit :: Hemopexin ELISA Kit

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Catalog # MBS564072
Unit / Price
  1x96 Wells  /  $480 +1 FREE 8GB USB
HPX elisa kit
Product Name

Hemopexin (HPX), ELISA Kit

Also Known As

Mouse Hemopexin ELISA Kit

Product Synonym Names
Mouse Hemopexin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
chromosome: 11; Location: 11p15.5-p15.4
OMIM
142290
3D Structure
ModBase 3D Structure for P02790
Host
Mouse
Samples
Immunoperoxidase Assay for Determination of Hemopexin in Mouse Samples
Assay Type
Sandwich
Intended Uses
The Hemopexin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin in serum, plasma of mice.
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HPX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHPXpurchase
MBS564072 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mouse Hemopexin (HPX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HPX. The ELISA analytical biochemical technique of the MBS564072 kit is based on HPX antibody-HPX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HPX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HPX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HPX elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Hemopexin (HX) is the plasma protein with the highest binding affinity to heme among known proteins. It is mainly expressed in liver and belongs to acute phase reactants, the synthesis of which is induced after inflammation. This ELISA kit can be used to measure Hemopexin in serum, tissue extracts and other biological fluids.
Product Categories/Family for HPX elisa kit
Sample Manual Insert of MBS564072. Click to request current manual
NCBI/Uniprot data below describe general gene information for HPX. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,676 Da[Similar Products]
NCBI Official Full Name
hemopexin
NCBI Official Synonym Full Names
hemopexin
NCBI Official Symbol
NCBI Official Synonym Symbols
HX; FLJ56652; HPX
  [Similar Products]
NCBI Protein Information
hemopexin; beta-1B-glycoprotein
UniProt Protein Name
Hemopexin
UniProt Synonym Protein Names
Beta-1B-glycoprotein
Protein Family
UniProt Gene Name
UniProt Entry Name
HEMO_HUMAN
NCBI Summary for HPX
This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq]
UniProt Comments for HPX
HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; metal ion binding; heme transporter activity

Biological Process: positive regulation of humoral immune response mediated by circulating immunoglobulin; receptor-mediated endocytosis; positive regulation of tyrosine phosphorylation of Stat1 protein; heme transport; positive regulation of immunoglobulin production; viral reproduction; cellular iron ion homeostasis; hemoglobin metabolic process; heme metabolic process
Research Articles on HPX
1. Results indicate that for the discrimination of AD from ND control subjects, measurement of a set of markers including Abeta1-42, ApoA1 and HPX improved diagnostic performance over that obtained by measurement of Abeta1-42 alone.
2. In a family with a hypomaturation-type enamel defect, mutational and haplotype analyses revealed an amelogenesis imperfecta-causing point mutation in exon 6 of MMP20 that results in a single amino acid substitution in the hemopexin domain.
3. these observations result from the binding of heme in form beta with an orientation that differs from the crystallographically observed binding orientation for rabbit hemopexin
4. The structural consequences of metal ion binding to the form of hemopexin that dominates in plasma have been evaluated.
5. Hemopexin in minimal change nephropathy relapse subjects may exist in an altered isoform, showing enhanced protease activity as compared with subjects in remission, subjects with other forms of primary glomerulopathy, or healthy controls.
6. HPX-heme(II)-NO appears to act as an efficient scavenger of peroxynitrite and of strong oxidants and nitrating species following the reaction of peroxynitrite with CO2
7. The conserved binding ability of the Hemopexin domains suggests that CD44 may act as a core molecule assembling multiple Membrane-type 1 matrix metalloproteinases on the cell surface.
8. Thermal denaturation of the human hemopexin-heme complex is investigated under a variety of solution conditions to identify factors that influence heme release, including the potential presence of transition metal ions or heme iron reduction.
9. The ability of hemopexin to bind metal ions raises the possibility that this protein may participate in the transport of metal ions in blood or in the exchange of metal ions between proteins.
10. Hemopexin and hemopexin domains of human proteins fulfill functions in activation of MMPs, inhibition of MMPs, dimerization, binding of substrates or ligands, cleavage of substrates, and endocytosis by LRP-1 and LRP-2.
11. hemopexin molecule as such can potentially act as a toxic protease, leading to proteinuria and glomerular alterations
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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