View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List HRE cell line Catalog # MBS668854 Unit / Price 1 Vial / $1,970 +2 FREE 8GB USB 5x1 Vial / $8,650 +10 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name HRE, Cell Line Popular Item Full Product Name HRE Leeporter HeLa Cell Line Product Gene Name HRE cell line[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Request for Current Manual Insert Request Current Manual Form/Format Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO. Dry Ice Shipment Extra charge fee may add to your shipping cost as dry ice is required to ship this product. Preparation and Storage Immediately upon receipt, store in liquid nitrogen.Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries. Other Notes Small volumes of HRE cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for HRE cell line The HRE Leeporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the hypoxia response element (HRE). In response to hypoxia (low oxygen), HREs of target genes are recognized and regulated by the hypoxia-inducible factors (HIFs) which belong to the family of basic helix-loop-helix transcription factors and form heterodimeric complex comprising the alpha subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3), among which HIF-1 alpha and HIF-2 alpha are predominant isoforms. Activation of HIFs can also be mediated by chemical hydroxylase inhibitors as hypoxia mimetics including the iron chelator desferrioxamine and cobalt chloride. Product Categories/Family for HRE cell line Cell Lines; Reporter Cell Lines Applications Tested/Suitable for HRE cell line Functional Assay Application Notes for HRE cell line Monitor the HIF induction activity. Screen for activators or inhibitors of the hypoxia signaling pathway. Culture conditions: Cells should be grown at 37?C with 5% CO2 using DMEM medium supplemented with 10% FBS and 1% Pen/Strep, plus 3 ug/ml of Puromycin. It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C-CO2 incubator. Leave the T25 flask in the incubator for 2~4 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1: 10 to 1: 20 weekly. Functional validation: A. Response of HRE Leeporter- HeLa cells to cobalt chloride (C degree Cl2). 1. Harvest HRE Leeporter- HeLa cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 10^4 cells/well. 2. Incubate cells at 37 degree C in a CO2 incubator for overnight. 3. The next day, stimulate cells with various concentrations of C degree Cl2. 4. Incubate at 37 degree C in a CO2 incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. Testing Data of HRE cell line Fig-1: Induction of HRE activity by cobalt chloride in HRE Leeporter HeLa cells. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP