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HRGP elisa kit :: Rat Histidine Rich Glycoprotein (HRGP) ELISA Kit

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Catalog # MBS263011
Unit / Price
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  48-Strip-Wells  /  $275 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Histidine Rich Glycoprotein (HRGP), ELISA Kit

Popular Item
Full Product Name

Rat Histidine Rich Glycoprotein (HRGP) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 613116
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HRGP concentration
Assay Type
Sandwich
Detection Range
200 ng/ml-3.12 ng/ml
Sensitivity
0.6 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HRGP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HRGPpurchase
MBS263011 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histidine Rich Glycoprotein (HRGP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HRGP. The ELISA analytical biochemical technique of the MBS263011 kit is based on HRGP antibody-HRGP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HRGP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HRGP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HRGP elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat HRGP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of HRGP elisa kit
HRGP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS263011. Click to request current manual
NCBI/Uniprot data below describe general gene information for HRGP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,578 Da
NCBI Official Full Name
histidine-rich glycoprotein
NCBI Official Synonym Full Names
histidine-rich glycoprotein
NCBI Official Symbol
NCBI Official Synonym Symbols
HPRG; HRGP; THPH11
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NCBI Protein Information
histidine-rich glycoprotein; histidine-proline-rich glycoprotein
UniProt Protein Name
Histidine-rich glycoprotein
UniProt Synonym Protein Names
Histidine-proline-rich glycoprotein; HPRG
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
HRG_HUMAN
NCBI Summary for HRGP
This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq, Jul 2008]
UniProt Comments for HRGP
HRG: Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in an heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis. Defects in HRG are the cause of thrombophilia due to histidine-rich glycoprotein deficiency (THPH11). A hemostatic disorder characterized by a tendency to thrombosis.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; heparan sulfate proteoglycan binding; heparin binding; protein binding; zinc ion binding; metal ion binding; heme binding; immunoglobulin binding; cysteine protease inhibitor activity; receptor binding

Biological Process: platelet activation; positive regulation of apoptosis; positive regulation of immune response to tumor cell; negative regulation of cell adhesion; chemotaxis; negative regulation of blood vessel endothelial cell migration; response to organic cyclic substance; negative regulation of cell proliferation; fibrinolysis; negative regulation of angiogenesis; platelet degranulation; negative regulation of fibrinolysis; positive regulation of focal adhesion formation; regulation of gene expression; regulation of actin cytoskeleton organization and biogenesis; regulation of blood coagulation; regulation of protein complex assembly; negative regulation of cell growth; angiogenesis; blood coagulation; regulation of peptidyl-tyrosine phosphorylation; defense response to fungus; negative regulation of cell adhesion mediated by integrin

Disease: Thrombophilia Due To Histidine-rich Glycoprotein Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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