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HSD11B1 elisa kit :: Porcine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit

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Catalog # MBS7247817
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
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  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
HSD11B1 elisa kit
Product Name

11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) (HSD11B1), ELISA Kit

Also Known As

Porcine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit

Product Synonym Names
Porcine 11 beta hydroxysteroid dehydrogenase type 1 (11b-HSD1) ELISA Kit
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 604931
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSD11B1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHSD11B1purchase
MBS7247817 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) (HSD11B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSD11B1. The ELISA analytical biochemical technique of the MBS7247817 kit is based on HSD11B1 antibody-HSD11B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD11B1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD11B1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for HSD11B1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
32,401 Da[Similar Products]
NCBI Official Full Name
11-beta hydroxysteroid dehydrogenase type 1
NCBI Official Synonym Full Names
hydroxysteroid (11-beta) dehydrogenase 1
NCBI Official Symbol
HSD11B1  [Similar Products]
NCBI Official Synonym Symbols
HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1
  [Similar Products]
NCBI Protein Information
corticosteroid 11-beta-dehydrogenase isozyme 1; short chain dehydrogenase/reductase family 26C, member 1
UniProt Protein Name
Corticosteroid 11-beta-dehydrogenase isozyme 1
UniProt Synonym Protein Names
11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1
UniProt Gene Name
HSD11B1  [Similar Products]
UniProt Synonym Gene Names
HSD11; HSD11L; 11-DH; 11-beta-HSD1  [Similar Products]
UniProt Entry Name
DHI1_HUMAN
NCBI Summary for HSD11B1
The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011]
UniProt Comments for HSD11B1
HSD11B1: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol. Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; Endoplasmic reticulum; EC 1.1.1.146; Membrane protein, integral; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 1q32-q41

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity

Biological Process: steroid metabolic process; glucocorticoid biosynthetic process; lung development

Disease: Cortisone Reductase Deficiency 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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