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HSD11b2 recombinant protein :: 11-Beta-Hydroxysteroid Dehydrogenase Type 2 Recombinant Protein

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Catalog # MBS2031421 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $265 +1 FREE 8GB USB
  0.1 mg  /  $415 +1 FREE 8GB USB
  0.2 mg  /  $510 +1 FREE 8GB USB
  0.5 mg  /  $990 +1 FREE 8GB USB
  1 mg  /  $1,465 +1 FREE 8GB USB
Product Name

11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2), Recombinant Protein

Popular Item
Full Product Name

Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
218030
Host
E.coli
Purity/Purification
> 90%
Concentration
Original Concentration: 200 ug/mL (lot specific)
Organism Species
Rattus norvegicus (Rat)
Source
Prokaryotic expression
Residues
Leu58~Pro393
Tags
N-terminal His Tag
Subcellular Location
Endoplasmic reticulum lumen
Traits
Freeze-dried powder
Buffer
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose
Predicted isoelectric point
9.2
Predicted Molecular Mass
40.5kDa
Accurate Molecular Mass
41kDa as determined by SDS-PAGE reducing conditions.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notEe vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of HSD11b2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for HSD11b2 recombinant protein
Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Sequence Information of HSD11b2 recombinant protein
HSD11b2 recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for HSD11b2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
11-beta-hydroxysteroid dehydrogenase type 2
NCBI Official Synonym Full Names
hydroxysteroid (11-beta) dehydrogenase 2
NCBI Official Symbol
HSD11B2  [Similar Products]
NCBI Official Synonym Symbols
AME; AME1; HSD2; HSD11K; SDR9C3
  [Similar Products]
NCBI Protein Information
corticosteroid 11-beta-dehydrogenase isozyme 2
UniProt Protein Name
Corticosteroid 11-beta-dehydrogenase isozyme 2
UniProt Synonym Protein Names
11-beta-hydroxysteroid dehydrogenase type 2; 11-DH2; 11-beta-HSD2; 11-beta-hydroxysteroid dehydrogenase type II; 11-HSD type II; 11-beta-HSD type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; 11-beta-HSD; Short chain dehydrogenase/reductase family 9C member 3
UniProt Gene Name
HSD11B2  [Similar Products]
UniProt Synonym Gene Names
; 11-DH2; 11-beta-HSD2; 11-HSD type II; 11-beta-HSD type II; 11-beta-HSD  [Similar Products]
UniProt Entry Name
DHI2_HUMAN
NCBI Summary for HSD11b2
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]
UniProt Comments for HSD11b2
HSD11B2: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). An autosomal recessive form of low-renin hypertension. It is usually diagnosed within the first years of life and is characterized by polyuria and polydipsia, failure to thrive, hypernatremia, severe hypertension with low renin and aldosterone levels, profound hypokalemia with metabolic alkalosis, and most often nephrocalcinosis. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Oxidoreductase; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; EC 1.1.1.-

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: endoplasmic reticulum

Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity; NAD binding; steroid binding

Biological Process: response to drug; response to food; response to glucocorticoid stimulus; response to hypoxia; aldosterone mediated regulation of blood volume; female pregnancy; glucocorticoid biosynthetic process; response to insulin stimulus

Disease: Apparent Mineralocorticoid Excess
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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