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HSD17b12 recombinant protein :: 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12) Recombinant Protein

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Catalog # MBS2011648 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $145 +1 FREE 8GB USB
  0.05 mg  /  $275 +1 FREE 8GB USB
  0.1 mg  /  $430 +1 FREE 8GB USB
  0.2 mg  /  $525 +1 FREE 8GB USB
  0.5 mg  /  $1,015 +1 FREE 8GB USB
  1 mg  /  $1,515 +1 FREE 8GB USB
SDS-Page
Product Name

17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12), Recombinant Protein

Full Product Name

Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -MECAPPAAG F LYWVGASTIA YLALRASYSL FRAFQVWCVG NEALVGPRLG EWAVVTGGTD GIGKAYAEEL AKRGMKIVLI SRSQDKLNQV SNNIKEKFNV ETRTIAVDFS LDDIYDKIKT GLSGLEIGVL VNNVGMSYEY PEYFLEIPDL DNTIKKLINI NVLSVCKVTR LVLPGMVERS KGVILNISSA SGMLPVPLLT IYSATKAFVD FFSQCLHEEY KSKGIFVQSV MPYLVATKLA KIQKPTLDKP SAETFVKSAI KTVGLQTRTT GYVIHSLMGS INSIMPRWMY FKIIMGFSKS LRNRYLKKRK KN
Chromosome Location
Chromosome: 2; NC_000068.7 (94032697..94157909, complement). Location: 2 E1; 2
3D Structure
ModBase 3D Structure for O70503
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Met1~Asn312 (Accession # O70503) with two N-terminal Tags, His-tag and GST-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of HSD17b12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HSD17b12 recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for HSD17b12 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

NCBI/Uniprot data below describe general gene information for HSD17b12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61.8kDa
NCBI Official Full Name
estradiol 17-beta-dehydrogenase 12
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 12
NCBI Official Symbol
Hsd17b12  [Similar Products]
NCBI Official Synonym Symbols
Kik1; KIK-I; AI172963; 2610510O05Rik
  [Similar Products]
NCBI Protein Information
estradiol 17-beta-dehydrogenase 12; KAR; keratonectin; keratoadhesin; 17-beta-HSD 12; steroid dehydrogenase; 3-ketoacyl-CoA reductase; 17-beta-hydroxysteroid dehydrogenase 12
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 12
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase (EC:1.3.1.-); KAR; KIK-I
UniProt Gene Name
Hsd17b12  [Similar Products]
UniProt Synonym Gene Names
Kik1; 17-beta-HSD 12; KAR  [Similar Products]
UniProt Entry Name
DHB12_MOUSE
UniProt Comments for HSD17b12
HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; EC 1.1.1.62; Membrane protein, integral; Oxidoreductase; Lipid Metabolism - androgen and estrogen; Membrane protein, multi-pass

Cellular Component: proteinaceous extracellular matrix; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: heparin binding; estradiol 17-beta-dehydrogenase activity; cis-chlorobenzene dihydrodiol dehydrogenase activity; cinnamate reductase activity; oxidoreductase activity; cis-2,3-dihydrodiol DDT dehydrogenase activity; collagen binding; 2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity; trans-1,2-dihydrodiolphenanthrene dehydrogenase activity; fibronectin binding; trans-9R,10R-dihydrodiolphenanthrene dehydrogenase activity; enoyl-[acyl-carrier-protein] reductase activity; 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase activity

Biological Process: extracellular matrix organization and biogenesis; metabolic process; lipid metabolic process; steroid biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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