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HSD17B3 elisa kit :: Human Testosterone ELISA Kit

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Catalog # MBS265979
Unit / Price
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  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Testosterone (HSD17B3), ELISA Kit

Popular Item
Full Product Name

Human Testosterone ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605573
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Testosterone concentration
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
0.05 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSD17B3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HSD17B3purchase
MBS265979 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Testosterone (HSD17B3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSD17B3. The ELISA analytical biochemical technique of the MBS265979 kit is based on HSD17B3 antibody-HSD17B3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD17B3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD17B3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HSD17B3 elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human Testosterone monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of HSD17B3 elisa kit
HSD17B3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for HSD17B3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
34,516 Da[Similar Products]
NCBI Official Full Name
testosterone 17-beta-dehydrogenase 3
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 3
NCBI Official Symbol
HSD17B3  [Similar Products]
NCBI Official Synonym Symbols
EDH17B3; SDR12C2
  [Similar Products]
NCBI Protein Information
testosterone 17-beta-dehydrogenase 3; 17-beta-HSD3; 17-beta-HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 12C, member 2
UniProt Protein Name
Testosterone 17-beta-dehydrogenase 3
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
UniProt Gene Name
HSD17B3  [Similar Products]
UniProt Synonym Gene Names
EDH17B3; 17-beta-HSD 3  [Similar Products]
UniProt Entry Name
DHB3_HUMAN
NCBI Summary for HSD17B3
This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]
UniProt Comments for HSD17B3
HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.1.1.64

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: testosterone 17-beta-dehydrogenase (NADP+) activity

Biological Process: steroid metabolic process; male genitalia development; androgen biosynthetic process

Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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