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HSD17B6 elisa kit :: Guinea Pig 17-beta-hydroxysteroid dehydrogenase type 6 (HSD17B6) ELISA Kit

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Catalog # MBS7238850
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HSD17B6 elisa kit
Product Name

17-beta-hydroxysteroid dehydrogenase type 6 (HSD17B6), ELISA Kit

Full Product Name

Guinea pig 17-beta-hydroxysteroid dehydrogenase type 6 (HSD17B6) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
606623
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSD17B6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHSD17B6purchase
MBS7238850 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 17-beta-hydroxysteroid dehydrogenase type 6 (HSD17B6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSD17B6. The ELISA analytical biochemical technique of the MBS7238850 kit is based on HSD17B6 antibody-HSD17B6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD17B6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD17B6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for HSD17B6 elisa kit
NCBI/Uniprot data below describe general gene information for HSD17B6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,966 Da[Similar Products]
NCBI Official Full Name
17-beta-hydroxysteroid dehydrogenase type 6
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 6
NCBI Official Symbol
HSD17B6  [Similar Products]
NCBI Official Synonym Symbols
HSE; RODH; SDR9C6
  [Similar Products]
NCBI Protein Information
17-beta-hydroxysteroid dehydrogenase type 6; 17-beta-HSD 6; oxidoreductase; 3-alpha->beta-HSE; retinol dehydrogenase; 3-hydroxysteroid epimerase; 3-alpha->beta-hydroxysteroid epimerase; 3(alpha->beta)-hydroxysteroid epimerase; 3(alpha->beta)-hydroxysteroid epimerasel; oxidative 3-alpha hydroxysteroid dehydrogenase; oxidative 3-alpha-hydroxysteroid-dehydrogenase; hydroxysteroid (17-beta) dehydrogenase 6 homolog; short chain dehydrogenase/reductase family 9C, member 6; NAD+ -dependent 3 alpha-hydroxysteroid dehydrogenase 3-hydroxysteroid epimerase
UniProt Protein Name
17-beta-hydroxysteroid dehydrogenase type 6
UniProt Synonym Protein Names
3-alpha->beta-hydroxysteroid epimerase; 3-alpha->beta-HSE; Oxidative 3-alpha hydroxysteroid dehydrogenase
UniProt Gene Name
HSD17B6  [Similar Products]
UniProt Synonym Gene Names
RODH; 17-beta-HSD 6; 17-beta-HSD6; 3-alpha->beta-HSE  [Similar Products]
UniProt Entry Name
H17B6_HUMAN
NCBI Summary for HSD17B6
The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. [provided by RefSeq, Aug 2013]
UniProt Comments for HSD17B6
HSD17B6: NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3- alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha- androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: EC 1.1.1.239; Oxidoreductase; EC 1.1.1.62; EC 1.1.1.105

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: endoplasmic reticulum; early endosome membrane; intracellular

Molecular Function: estradiol 17-beta-dehydrogenase activity; electron carrier activity; oxidoreductase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; retinol dehydrogenase activity

Biological Process: androgen biosynthetic process; androgen catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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