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HSD3B1 elisa kit :: Human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit

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Catalog # MBS9428371
Unit / Price
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  96 Tests  /  $575 +1 FREE 8GB USB
HSD3B1 elisa kit
Product Name

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (HSD3B1), ELISA Kit

Full Product Name

Human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit

Product Synonym Names
3 beta-hydroxysteroid dehydrogenase/Delta 5--> 4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5--> 4-isomerase type I; 3-beta-HSD I; Trophoblast antigen
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
M27137 mRNA
3D Structure
ModBase 3D Structure for P14060
Species Reactivity
Samples
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.312-20 ng/mL
Sensitivity
Less than 0.19ng/mL
Assay Time
3-5h
Sample Volume
50T
Detection Wavelength
450 nm
Preparation and Storage
Store at 4 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSD3B1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HSD3B1purchase
MBS9428371 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (HSD3B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSD3B1. The ELISA analytical biochemical technique of the MBS9428371 kit is based on HSD3B1 antibody-HSD3B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD3B1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD3B1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for HSD3B1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
42,252 Da
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Synonym Protein Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; Trophoblast antigen FDO161GIncluding the following 2 domains:3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)Alternative name(s):3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase
UniProt Gene Name
HSD3B1  [Similar Products]
UniProt Synonym Gene Names
3BH; HSDB3A; 3-beta-HSD I  [Similar Products]
UniProt Comments for HSD3B1
HSD3B1: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. Belongs to the 3-beta-HSD family.

Protein type: EC 1.1.1.145; EC 5.3.3.1; Isomerase; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: endoplasmic reticulum membrane; mitochondrial inner membrane; mitochondrial intermembrane space; smooth endoplasmic reticulum membrane

Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; steroid delta-isomerase activity

Biological Process: androgen biosynthetic process; estrogen biosynthetic process; glucocorticoid biosynthetic process; mineralocorticoid biosynthetic process; steroid biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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