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HSP-70 elisa kit :: Dog Heat Shock Protein 70 (HSP-70) ELISA Kit

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Catalog # MBS705544
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Heat Shock Protein 70 (HSP-70), ELISA Kit

Popular Item
Also Known As

Canine Heat Shock Protein 70, HSP-70 ELISA Kit

Product Synonym Names
Canine Heat Shock Protein 70 (HSP-70) ELISA Kit; Heat Shock Protein 70 (HSP-70)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS705544 Testing Data
Sequence Length
608
3D Structure
ModBase 3D Structure for Q40058
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Dog HSP-70. No significant cross-reactivity or interference between Dog HSP-70 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
1.25 ng/ml-80 ng/ml
Sensitivity
0.312 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSP-70 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHSP-70purchase
MBS705544 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heat Shock Protein 70 (HSP-70) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSP-70. The ELISA analytical biochemical technique of the MBS705544 kit is based on HSP-70 antibody-HSP-70 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSP-70 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSP-70. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HSP-70 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSP-70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP-70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP-70 is added to the wells. After washing, avidin conjugated Canineradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP-70 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of HSP-70 elisa kit
HSP-70 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS705544. Click to request current manual
NCBI/Uniprot data below describe general gene information for HSP-70. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
67,016 Da
NCBI Official Full Name
HSP70
UniProt Protein Name
HSP70
UniProt Synonym Protein Names
HSP70
UniProt Entry Name
Q40058_HORVU
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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