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HSP-72 elisa kit :: Chicken Heat Shock Protein 72, HSP-72 ELISA Kit

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Catalog # MBS705243
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Heat Shock Protein 72, HSP-72, ELISA Kit

Also Known As

Chicken Heat Shock Protein 72, HSP-72 ELISA Kit

Product Synonym Names
Chicken Heat Shock Protein 72; HSP-72 ELISA Kit; Heat Shock Protein 72; HSP-72
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS705243 Testing Data
Sequence Length
151
OMIM
gene 603012
3D Structure
ModBase 3D Structure for P08107
Species Reactivity
Samples
Serum, plasma and tissue homogenates
Assay Type
Competitive
Detection Range
1.25 ng/ml-80 ng/ml
Sensitivity
0.31 ng/ml
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSP-72 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHSP-72purchase
MBS705243 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heat Shock Protein 72, HSP-72, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSP-72. The ELISA analytical biochemical technique of the MBS705243 kit is based on HSP-72 antibody-HSP-72 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSP-72 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSP-72. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HSP-72 elisa kit
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. An antibody specific for HSP-72 has been pre-coated onto a microplate. Add HSP-72 (Standards or samples) to the well, and then add Biotin-conjugated HSP-72. A competitive inhibition reaction is launched between HSP-72 (Standards or samples) and Biotin conjugated HSP-72 with the HSP-72 antibody. Then add the HRP-avidin to each well. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of HSP-72 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of HSP-72 elisa kit
HSP-72 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS705243. Click to request current manual
NCBI/Uniprot data below describe general gene information for HSP-72. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
63,937 Da
NCBI Official Full Name
heat shock protein 72, partial
NCBI Official Synonym Full Names
heat shock 70kDa protein 1A
NCBI Official Symbol
HSPA1A  [Similar Products]
NCBI Official Synonym Symbols
HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103
  [Similar Products]
NCBI Protein Information
heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; epididymis secretory protein Li 103; dnaK-type molecular chaperone HSP70-1
UniProt Protein Name
Heat shock 70 kDa protein 1A/1B
UniProt Synonym Protein Names
Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2
UniProt Gene Name
HSPA1A  [Similar Products]
UniProt Synonym Gene Names
HSPA1; HSX70
HSPA1B; HSP70-1/HSP70-2; HSP70.1/HSP70.2  [Similar Products]
UniProt Entry Name
HSP71_HUMAN
NCBI Summary for HSP-72
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
UniProt Comments for HSP-72
Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.1 PublicationManual assertion based on experiment in:Ref.20
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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