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HSP60 recombinant protein

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Catalog # MBS203138
Unit / Price
  0.1 mg  /  $310 +1 FREE 8GB USB
  0.5 mg  /  $720 +1 FREE 8GB USB
SDS-Page
Product Name

HSP60, Recombinant Protein

Popular Item
Full Product Name

HSP60, 1-573aa, Human, His tag, E Coli

Product Synonym Names
Heat shock 60kDa protein 1; CPN60; GroEL; HSP65; SPG13; HuCHA60; Heat shock 60kDa protein 1; HSP60; Heat shock 60kDa protein 1 60 kDa chaperonin; GroEL; E; coli; homolog of; 60 kDa heat shock protein mitochondrial; 60kDa; cb863; Chaperonin; Chaperonin 60; Chaperonin; 60-KD; CPN 60; fa04a05; fb22d10; fi27b05; GroEL Homolog; Heat shock 60kD protein 1 (chaperonin); Heat shock 60kD protein 1 chaperonin; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 1 (chaperonin); Heat Shock Protein 60; Heat shock protein 65; HLD4; Hsp 60; HSP 65; HSPD 1; HSPD1; HuCHA60; id:ibd2197; Spastic paraplegia 13 Mitochondrial matrix protein P1; P60 lymphocyte protein; sb:cb144; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (autosomal dominant); SPG 13; wu:fa04a05; wu:fb22d10; wu:fi04a12; wu:fi27b05.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALM LQGVDLLADA VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQD VANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA VIAELKKQSK PVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP KEEKDPGMGA MGGMGGGMGG GMF
OMIM
M22382 mRNA
3D Structure
ModBase 3D Structure for P10809
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 25mM Tris pH 7.5, 100mM NaCl, 5mM DTT, 10% glycerol.
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of HSP60 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HSP60 recombinant protein
Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E Coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding. Recombinant human HSP60, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for HSP60 recombinant protein
Applications Tested/Suitable for HSP60 recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for HSP60. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
63kDa (593aa)
NCBI Official Full Name
60 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein family D (Hsp60) member 1
NCBI Official Symbol
HSPD1  [Similar Products]
NCBI Official Synonym Symbols
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
  [Similar Products]
NCBI Protein Information
60 kDa heat shock protein, mitochondrial
UniProt Protein Name
60 kDa heat shock protein, mitochondrial
UniProt Synonym Protein Names
60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
Protein Family
UniProt Gene Name
HSPD1  [Similar Products]
UniProt Synonym Gene Names
HSP60; CPN60; HSP-60; Hsp60  [Similar Products]
NCBI Summary for HSP60
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
UniProt Comments for HSP60
Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Product References and Citations for HSP60 recombinant protein
Cheng MY., et al. (1990), Nature. 348: 455- 458; Ghosh JC., et al. (2008), J Biol Chem. Feb 22; 283 (8):5188-94

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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