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HSP90 alpha recombinant protein

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Catalog # MBS515368
Unit / Price
  0.02 mg  /  $260 +1 FREE 8GB USB
  0.05 mg  /  $415 +1 FREE 8GB USB
SDS-PAGE
Product Name

HSP90 alpha, Recombinant Protein

Popular Item
Also Known As

HSP90 alpha recombinant protein

Product Synonym Names
HSP90AA1; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP90N; HSPCAL1; HSPCAL4; FLJ31884; HSP90alpha Protein; HSP90a Protein
Product Synonym Gene Name
HSP90AA1; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP90N; HSPCAL1; HSPCAL4; FLJ31884[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
3366
Chromosome Location
Chromosome: 14; NC_000014.8 (102547075..102606086, complement). Location: 14q32.33
OMIM
140571
3D Structure
ModBase 3D Structure for P07900
Host
Sf9 insect cells
Purity/Purification
>90%
Form/Format
50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Concentration
0.2ug/ul (lot specific)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full length human HSP90alpha was expressed by baculovirus in Sf9 insect cells
Accession Number
NM 005348
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Stability: 1yr at -70 degree C from date of shipment.
Other Notes
Small volumes of HSP90 alpha recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HSP90 alpha recombinant protein
Recombinant full-length human HSP90 alpha was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag.

Scientific Background: HSP90α is a member of the HSP90 family of proteins which are important molecular chaperones involved in signal transduction, cell cycle control, stress management, folding, degradation, and transport of proteins (1). HSP90 is a molecular chaperone that plays a key role in the conformational maturation of oncogenic signaling proteins, including HER2/ERBB2, AKT, RAF1, BCR-ABL and mutated p53. HSP90 inhibitors bind to HSP90, and induce the proteasomal degradation of HSP90 client proteins. HSP90α is an important mediator of cancer cell invasion and is expressed extracellularly on fibrosarcoma and breast cancer cells where it interacts with MMP2 (2).
Applications Tested/Suitable for HSP90 alpha recombinant protein
Western Blot (WB)

The purity was determined to be >90% by densitometry . Approx. MW 94 kDa
HSP90 alpha recombinant protein SDS-PAGE image
NCBI/Uniprot data below describe general gene information for HSP90 alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
~94 kDa
NCBI Official Full Name
Homo sapiens heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2, mRNA
NCBI Official Synonym Full Names
heat shock protein 90kDa alpha (cytosolic), class A member 1
NCBI Official Symbol
HSP90AA1  [Similar Products]
NCBI Official Synonym Symbols
EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
  [Similar Products]
NCBI Protein Information
heat shock protein HSP 90-alpha; HSP 86; heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kDa protein 1, alpha; renal carcinoma antigen NY-REN-38; heat shock 90kD protein, alpha-like 4; epididymis luminal secretory protein 52; heat shock 90kD protein 1, alpha-like 4
UniProt Protein Name
Heat shock protein HSP 90-alpha
UniProt Synonym Protein Names
Heat shock 86 kDa; HSP 86; HSP86; Renal carcinoma antigen NY-REN-38
UniProt Gene Name
HSP90AA1  [Similar Products]
UniProt Synonym Gene Names
HSP90A; HSPC1; HSPCA; HSP 86; HSP86  [Similar Products]
UniProt Entry Name
HS90A_HUMAN
NCBI Summary for HSP90 alpha
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Comments for HSP90 alpha
Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Ref.15 Ref.22 Ref.28

Subunit structure: Homodimer. Identified in NR3C1/GCR steroid receptor-chaperone complexes formed at least by NR3C1, HSP90AA1 and a variety of proteins containing TPR repeats such as FKBP4, FKBP5, PPID, PPP5C STIP1. Interacts with AHSA1, FNIP1, HSF1, SMYD3 and TOM34. Interacts with TERT; the interaction, together with PTGES3, is required for correct assembly and stabilization of the TERT holoenzyme complex. Interacts with CHORDC1 and DNAJC7. Interacts with STUB1 and UBE2N; may couple the chaperone and ubiquitination systems. Interacts (via TPR repeat-binding motif) with PPP5C (via TPR repeats); the interaction is direct and activates PPP5C phosphatase activity. Ref.17 Ref.18 Ref.19 Ref.20 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.31 Ref.40 Ref.43 Ref.50 Ref.51

Subcellular location: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.42

Domain: The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.

Post-translational modification: ISGylated. Ref.41S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.

Sequence similarities: Belongs to the heat shock protein 90 family.
Product References and Citations for HSP90 alpha recombinant protein
1. Csermely, P. et al: The 90-kDa molecular chaperone family: structure, function, and clinical applications. Pharmacol Ther. 1998 Aug;79(2):129-68.
2. Eustace, B. K. et al:Functional proteomic screens reveal an essential extracellular role for hsp90-alpha in cancer cell invasiveness. Nature Cell Biol. 6: 507-514, 2004.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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