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HSP90AB1 elisa kit :: Mouse heat shock protein 90kDa alpha (cytosolic) , class B member 1 ELISA Kit

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Catalog # MBS921345
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

heat shock protein 90kDa alpha (cytosolic) , class B member 1 (HSP90AB1), ELISA Kit

Popular Item
Also Known As

Mouse Heat shock protein HSP 90-beta, HSP90AB1 ELISA Kit

Product Synonym Names
Mouse Heat shock protein HSP 90-beta (HSP90AB1) ELISA kit; RP1-302G2.1; D6S182; FLJ26984; HSP90-BETA; HSP90B; HSPC2; HSPCB; OTTHUMP00000016518; OTTHUMP00000016519; heat shock 90kD protein 1; beta; heat shock 90kDa protein 1; beta; heat shock protein beta; heat shock protein 90kDa alpha (cytosolic); class B member 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS921345 Testing Data
Sequence Length
724
3D Structure
ModBase 3D Structure for P11499
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse HSP90AB1. No significant cross-reactivity or interference between mouse HSP90AB1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/ml -1000 pg/ml
Sensitivity
Typically less than 3.9 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSP90AB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHSP90AB1purchase
MBS921345 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSP90AB1. The ELISA analytical biochemical technique of the MBS921345 kit is based on HSP90AB1 antibody-HSP90AB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSP90AB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSP90AB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HSP90AB1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSP90AB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP90AB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP90AB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP90AB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of HSP90AB1 elisa kit
HSP90AB1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS921345. Click to request current manual
NCBI/Uniprot data below describe general gene information for HSP90AB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
83,281 Da
NCBI Official Full Name
heat shock protein HSP 90-beta
NCBI Official Synonym Full Names
heat shock protein 90 alpha (cytosolic), class B member 1
NCBI Official Symbol
Hsp90ab1  [Similar Products]
NCBI Official Synonym Symbols
90kDa; Hsp84; Hsp90; Hspcb; C81438; Hsp84-1; AL022974
  [Similar Products]
NCBI Protein Information
heat shock protein HSP 90-beta; TSTA; HSP 84; heat shock 84 kDa; heat shock protein 1, beta; heat shock protein, 84 kDa 1; heat shock 90kDa protein 1, beta; retinal degeneration slow protein; tumor-specific transplantation 84 kDa antigen; heat shock protein 90kDa alpha (cytosolic), class B member 1
UniProt Protein Name
Heat shock protein HSP 90-beta
UniProt Synonym Protein Names
Heat shock 84 kDa; HSP 84; HSP84; Tumor-specific transplantation 84 kDa antigen; TSTA
Protein Family
UniProt Gene Name
Hsp90ab1  [Similar Products]
UniProt Synonym Gene Names
Hsp84; Hsp84-1; Hspcb; HSP 84; HSP84; TSTA  [Similar Products]
UniProt Entry Name
HS90B_MOUSE
UniProt Comments for HSP90AB1
HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Chaperone

Cellular Component: signalosome; cell surface; mitochondrion; membrane; brush border membrane; basolateral plasma membrane; apical plasma membrane; cytoplasm; intracellular; inclusion body; cytosol

Molecular Function: protein binding; GTP binding; double-stranded RNA binding; unfolded protein binding; dATP binding; nucleotide binding; sulfonylurea receptor binding; protein kinase binding; kinase binding; ATP binding

Biological Process: positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; protein folding; positive regulation of cell size; response to stress; negative regulation of neuron apoptosis; placenta development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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