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HSPA8 elisa kit :: Mouse heat shock 70kDa protein 8 ELISA Kit

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Catalog # MBS926334
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Typical Testing Data/Standard Curve (for reference only)
Product Name

heat shock 70kDa protein 8 (HSPA8), ELISA Kit

Popular Item
Full Product Name

Mouse Heat shock cognate 71 kDa protein, HSPA8 ELISA Kit

Product Synonym Names
Mouse Heat shock cognate 71 kDa protein (HSPA8) ELISA kit; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP1; MGC131511; MGC29929; NIP71; LPS-associated protein 1; N-myristoyltransferase inhibitor protein 71; constitutive heat shock protein 70; heat shock 70kD p; heat shock 70kDa protein 8
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS926334 Testing Data
3D Structure
ModBase 3D Structure for P63017
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse HSPA8. No significant cross-reactivity or interference between Mouse HSPA8 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
9.4 pg/ml-600 pg/ml
Sensitivity
2.35 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Neurobiology
Protein Biological Process 3
Stress response
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSPA8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHSPA8purchase
MBS926334 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heat shock 70kDa protein 8 (HSPA8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSPA8. The ELISA analytical biochemical technique of the MBS926334 kit is based on HSPA8 antibody-HSPA8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPA8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPA8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HSPA8 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPA8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPA8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPA8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPA8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of HSPA8 elisa kit
HSPA8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS926334. Click to request current manual
NCBI/Uniprot data below describe general gene information for HSPA8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
70,871 Da
NCBI Official Full Name
heat shock cognate 71 kDa protein
NCBI Official Synonym Full Names
heat shock protein 8
NCBI Official Symbol
Hspa8  [Similar Products]
NCBI Official Synonym Symbols
Hsc70; Hsc71; Hsc73; Hsp73; Hspa10; 2410008N15Rik
  [Similar Products]
NCBI Protein Information
heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock 70kD protein 8; heat shock 70 kDa protein 8; heat shock protein cognate 70
UniProt Protein Name
Heat shock cognate 71 kDa protein
UniProt Synonym Protein Names
Heat shock 70 kDa protein 8
UniProt Gene Name
Hspa8  [Similar Products]
UniProt Synonym Gene Names
Hsc70; Hsc73  [Similar Products]
UniProt Entry Name
HSP7C_MOUSE
UniProt Comments for HSPA8
HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone; Heat shock protein; RNA-binding; Nucleolus

Cellular Component: extracellular space; neuron projection; focal adhesion; protein complex; terminal button; ribonucleoprotein complex; cytosol; spliceosome; synaptic vesicle; membrane; cell soma; cytoplasm; plasma membrane; intracellular; nucleus; myelin sheath; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; ubiquitin protein ligase binding; ATPase activity; unfolded protein binding; nucleotide binding; ATPase activity, coupled; ADP binding; ATP binding; receptor binding

Biological Process: positive regulation of nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; protein folding; transcription, DNA-dependent; chaperone cofactor-dependent protein folding; regulation of cell cycle; RNA splicing; protein refolding; mRNA processing; negative regulation of transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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