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HSPG2 elisa kit :: Human Basement membrane-specific heparan sulfate proteoglycan core protein ELISA Kit

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Catalog # MBS765938
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Product Name

Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2), ELISA Kit

Also Known As

Human Basement membrane-specific heparan sulfate proteoglycan core protein ELISA Kit

Product Synonym Names
HSPG2/PLC/HSPG/PRCAN/SJA/SJS/SJS1/Perlecan/endorepellin (domain V region)/heparan sulfate proteoglycan 2/perlecan proteoglycan/PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)/
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
4391
OMIM
142461
Species Reactivity
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.625-40ng/ml
Sensitivity
0.375ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HSPG2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forHSPG2purchase
MBS765938 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSPG2. The ELISA analytical biochemical technique of the MBS765938 kit is based on HSPG2 antibody-HSPG2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPG2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPG2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HSPG2 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HSPG2antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HSPG2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HSPG2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman HSPG2can be calculated.

Typical Testing Data of HSPG2 elisa kit
HSPG2 elisa kit Typical Testing Data image
Typical Standard Curve of HSPG2 elisa kit
HSPG2 elisa kit Typical Standard Curve image
Sample Manual Insert of MBS765938. Click to request current manual
NCBI/Uniprot data below describe general gene information for HSPG2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
468,830 Da
NCBI Official Full Name
basement membrane-specific heparan sulfate proteoglycan core protein isoform b
NCBI Official Synonym Full Names
heparan sulfate proteoglycan 2
NCBI Official Symbol
HSPG2  [Similar Products]
NCBI Official Synonym Symbols
PLC; SJA; SJS; HSPG; SJS1; PRCAN
  [Similar Products]
NCBI Protein Information
basement membrane-specific heparan sulfate proteoglycan core protein
UniProt Protein Name
Basement membrane-specific heparan sulfate proteoglycan core protein
UniProt Synonym Protein Names
Perlecan; PLC
UniProt Gene Name
HSPG2  [Similar Products]
UniProt Synonym Gene Names
HSPG; PLC  [Similar Products]
UniProt Entry Name
PGBM_HUMAN
NCBI Summary for HSPG2
This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Comments for HSPG2
HSPG2: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1); a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH). The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.1-p34

Cellular Component: basal lamina; extracellular matrix; extracellular region; extracellular space; focal adhesion; Golgi lumen; lysosomal lumen; plasma membrane

Molecular Function: metal ion binding; protein binding; protein C-terminus binding

Biological Process: angiogenesis; brain development; carbohydrate metabolic process; cardiac muscle development; cellular protein metabolic process; chondrocyte differentiation; chondroitin sulfate metabolic process; embryonic skeletal morphogenesis; endochondral ossification; extracellular matrix disassembly; extracellular matrix organization and biogenesis; fat-soluble vitamin metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; lipoprotein metabolic process; phototransduction, visible light; protein localization; retinoid metabolic process; vitamin metabolic process

Disease: Dyssegmental Dysplasia, Silverman-handmaker Type; Schwartz-jampel Syndrome, Type 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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