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HSPG2 recombinant protein :: Heparan Sulfate Proteoglycan 2 (HSPG2) Recombinant Protein

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Catalog # MBS2011529
Unit / Price
  0.01 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $230 +1 FREE 8GB USB
  0.1 mg  /  $345 +1 FREE 8GB USB
  0.2 mg  /  $430 +1 FREE 8GB USB
  0.5 mg  /  $825 +1 FREE 8GB USB
  1 mg  /  $1,215 +1 FREE 8GB USB
Product Name

Heparan Sulfate Proteoglycan 2 (HSPG2), Recombinant Protein

Popular Item
Full Product Name

Recombinant Heparan Sulfate Proteoglycan 2 (HSPG2)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2011529 Technical Datasheet
Chromosome Location
Chromosome: 1; NC_000001.10 (22148737..22263750, complement). Location: 1p36.1-p34
OMIM
142461
3D Structure
ModBase 3D Structure for P98160
Host
E. coli
Purity/Purification
> 97%
Concentration
Original Concentration: 200 ug/mL (lot specific)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Leu4149~Ser4391
Tags
N-terminal His Tag
Subcellular Location
Secreted, Extracellular matrix
Traits
Freeze-dried powder
Buffer
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose
Predicted isoelectric point
6.0
Predicted Molecular Mass
27.0kDa
Accurate Molecular Mass
28kDa as determined by SDS-PAGE reducing conditions
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of HSPG2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for HSPG2 recombinant protein
Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Sequence Information of HSPG2 recombinant protein
HSPG2 recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for HSPG2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
basement membrane-specific heparan sulfate proteoglycan core protein
NCBI Official Synonym Full Names
heparan sulfate proteoglycan 2
NCBI Official Symbol
HSPG2  [Similar Products]
NCBI Official Synonym Symbols
PLC; SJA; SJS; HSPG; SJS1; PRCAN
  [Similar Products]
NCBI Protein Information
basement membrane-specific heparan sulfate proteoglycan core protein; perlecan proteoglycan; endorepellin (domain V region)
UniProt Protein Name
Basement membrane-specific heparan sulfate proteoglycan core protein
UniProt Synonym Protein Names
Perlecan
Protein Family
UniProt Gene Name
HSPG2  [Similar Products]
UniProt Synonym Gene Names
HSPG; PLC  [Similar Products]
UniProt Entry Name
PGBM_HUMAN
NCBI Summary for HSPG2
This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Comments for HSPG2
HSPG2: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1); a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH). The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

Protein type: Secreted; Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p36.1-p34

Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; focal adhesion; Golgi lumen; plasma membrane; extracellular region; basal lamina

Molecular Function: protein C-terminus binding; protein binding; metal ion binding

Biological Process: cardiac muscle development; phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; lipoprotein metabolic process; pathogenesis; embryonic skeletal morphogenesis; chondroitin sulfate metabolic process; extracellular matrix disassembly; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; protein localization; carbohydrate metabolic process; chondrocyte differentiation; angiogenesis; brain development; retinoid metabolic process; endochondral ossification

Disease: Schwartz-jampel Syndrome, Type 1; Dyssegmental Dysplasia, Silverman-handmaker Type
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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