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HTR1A cell line :: 5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A) Cell Line

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Catalog # MBS430276
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Product Name

5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A), Cell Line

Full Product Name

Human 5-hydroxytryptamine (serotonin) receptor 1A (HTR1A) ACTOne Stable Cell Line

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Cell Culture Medium
Growth medium (for HTR1A Stable Cell Line): 90% DMEM, 10% FBS, supplemented with 250 ug/ml G418, 1 ug/ml Puromycin
Growth medium (for Parental Cell Line): 90% DMEM, 10% FBS, supplemented with 250 ug/ml G418
Freezing medium: 10% DMSO, 90% FBS
Mycoplasma Contamination Test
This cell line has been tested negative for Mycoplasma sp.
Shipping Note
Product available only in the USA.
Growth Properties
Adherent
Detection
FlexStation or Microplate reader
Other Notes
Small volumes of HTR1A cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HTR1A cell line
Introduction: HTR1A, expressed in the pancreas and gastrointestinal tract, encodes a member of the rhodopsin subfamily of G-proteincoupled receptors. The encoded protein is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.
Description: Human HTR1A ACTOne is a HEK293-CNG cell line that expresses recombinant human HTR1A. HEK293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye (Cat# MBS430187). The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Application Notes for HTR1A cell line
cAMP dependent human HTR1A cell-based assay
cell-based high-throughput screening of human HTR1A agonists/antagonists.

Testing Data of HTR1A cell line
HTR1A cell line Testing Data image
Testing Data of HTR1A cell line
HTR1A cell line Testing Data image
NCBI/Uniprot data below describe general gene information for HTR1A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,107 Da
NCBI Official Synonym Full Names
5-hydroxytryptamine receptor 1A
NCBI Official Symbol
HTR1A  [Similar Products]
NCBI Official Synonym Symbols
G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
  [Similar Products]
NCBI Protein Information
5-hydroxytryptamine receptor 1A
UniProt Protein Name
5-hydroxytryptamine receptor 1A
UniProt Synonym Protein Names
G-21; Serotonin receptor 1A
UniProt Gene Name
HTR1A  [Similar Products]
UniProt Synonym Gene Names
ADRB2RL1; ADRBRL1; 5-HT-1A; 5-HT1A  [Similar Products]
NCBI Summary for HTR1A
This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]
UniProt Comments for HTR1A
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with HTR1A cell line
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >115 publications with HTR1A and Brain
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