• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

HTRA1 elisa kit :: Human HtrA serine peptidase 1 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS902316
Unit / Price
Scan QR to view Datasheet
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

HtrA serine peptidase 1 (HTRA1), ELISA Kit

Popular Item
Full Product Name

Human Serine protease HTRA1, HTRA1 ELISA Kit

Product Synonym Names
Human Serine protease HTRA1 (HTRA1) ELISA kit; ARMD7; HtrA; L56; ORF480; PRSS11; IGFBP5-protease; protease; serine; 11 (IGF binding) ; HtrA serine peptidase 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS902316 Testing Data
OMIM
gene 610149
3D Structure
ModBase 3D Structure for Q92743
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human HTRA1. No significant cross-reactivity or interference between human HTRA1 and analogues was observed.
Samples
Serum, plasma, cell culture supernates
Assay Type
Quantitative Sandwich
Detection Range
1.56 ng/ml -100 ng/ml
Sensitivity
Typically less than 0.39 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HTRA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HTRA1purchase
MBS902316 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HtrA serine peptidase 1 (HTRA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTRA1. The ELISA analytical biochemical technique of the MBS902316 kit is based on HTRA1 antibody-HTRA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTRA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTRA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HTRA1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HTRA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTRA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTRA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTRA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of HTRA1 elisa kit
HTRA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS902316. Click to request current manual
NCBI/Uniprot data below describe general gene information for HTRA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,287 Da
NCBI Official Full Name
serine protease HTRA1
NCBI Official Synonym Full Names
HtrA serine peptidase 1
NCBI Official Symbol
HTRA1  [Similar Products]
NCBI Official Synonym Symbols
L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL
  [Similar Products]
NCBI Protein Information
serine protease HTRA1; IGFBP5-protease; protease, serine, 11 (IGF binding); high-temperature requirement A serine peptidase 1
UniProt Protein Name
Serine protease HTRA1
UniProt Synonym Protein Names
High-temperature requirement A serine peptidase 1; L56; Serine protease 11
Protein Family
UniProt Gene Name
HTRA1  [Similar Products]
UniProt Synonym Gene Names
HTRA; PRSS11  [Similar Products]
UniProt Entry Name
HTRA1_HUMAN
NCBI Summary for HTRA1
This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]
UniProt Comments for HTRA1
HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: extracellular matrix; extracellular space; cytosol

Molecular Function: insulin-like growth factor binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of defense response to virus; regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; proteolysis; positive regulation of epithelial cell proliferation; negative regulation of BMP signaling pathway

Disease: Macular Degeneration, Age-related, 7; Cerebral Autosomal Recessive Arteriopathy With Subcortical Infarcts And Leukoencephalopathy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000