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Human Centromere B ELISA Kit

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Catalog # MBS494733-ORG 633
Unit / Price DISCONTINUED
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Centromere B elisa kit
Product Name

Centromere B, ELISA Kit

Popular Item
Also Known As

Anti-Centromere B ELISA

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
The microplate is coated with recombinant Centromer B as antigen.The Anti-Centromer B test kits recognises only autoantibodies specific to the concerning antigen. No crossreactivities to other autoantigens have been observed.
Samples
Human serum or plasma
Sensitivity
The lower detection limit for the Anti-Centromere B test was determined at 1.0 U/ml.
Intended Uses
Anti-Centromere B is an indirect solid phase enzyme immunoassay (ELISA) for the determination of IgG class autoantibodies against Centromere B in human serum or plasma. The assay is intended for research use only, not for use in diagnostic procedures.
Preparation and Storage
2 - 8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Centromere B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCentromere Bpurchase
MBS494733-ORG 633 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Centromere B, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Centromere B. The ELISA analytical biochemical technique of the MBS494733-ORG 633 kit is based on Centromere B antibody-Centromere B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Centromere B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Centromere B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Centromere B elisa kit
Principle of the assay: Recombinant centromere protein B is bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the respective antigen.Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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