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Human Mycobacterium Tuberculosis IgM ELISA Kit

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Catalog # MBS494348
Unit / Price
  96 Tests  /  $275 +1 FREE 8GB USB
Mycobacterium Tuberculosis IgM elisa kit
Product Name

Mycobacterium Tuberculosis IgM, ELISA Kit

Popular Item
Also Known As

Mycobacterium Tuberculosis IgM ELISA

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Samples
Serum and plasma
Preparation and Storage
2 - 8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Mycobacterium Tuberculosis IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMycobacterium Tuberculosis IgMpurchase
MBS494348 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mycobacterium Tuberculosis IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Mycobacterium Tuberculosis IgM. The ELISA analytical biochemical technique of the MBS494348 kit is based on Mycobacterium Tuberculosis IgM antibody-Mycobacterium Tuberculosis IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Mycobacterium Tuberculosis IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Mycobacterium Tuberculosis IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Mycobacterium Tuberculosis IgM elisa kit
Intended Uses: The Mycobacterium tuberculosis IgM antibody ELISA kit has been designed for the detection of specific IgM antibodies against Mycobacterium tuberculosis in serum and plasma. For research use only - Not for use in diagnostic procedures.

Principle of the Assay: The Mycobacterium tuberculosis IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Mycobacterium tuberculosis antigen is bound on the surface of the microtiter strips. Diluted serum or ready-to-use standards are plpetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Mycobacterium tuberculosis antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-hum an-19M peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentratIon of IgM antibodies is directly proportional to the intenSity of the color.
Sample Manual Insert of MBS494348. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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